Practical application of different enzymes immobilized on sepabeads

被引：62

作者：

Hilterhaus, L. ^{[1
]}

Minow, B. ^{[2
]}

Mueller, J. ^{[2
]}

Berheide, M. ^{[1
]}

Quitmann, H. ^{[2
]}

Katzer, M. ^{[3
]}

Thum, O. ^{[4
]}

Antranikian, G. ^{[3
]}

Zeng, A. P. ^{[2
]}

Liese, A. ^{[1
]}

机构：

[1] Inst Tech Biocatalysis, D-21073 Hamburg, Germany

[2] Inst Bioproc & Biosyst Engn, D-21073 Hamburg, Germany

[3] Inst Tech Microbiol, D-21073 Hamburg, Germany

[4] Evon Goldschmidt GmbH, D-45172 Essen, Germany

来源：

BIOPROCESS AND BIOSYSTEMS ENGINEERING | 2008年 / 31卷 / 03期

关键词：

immobilized enzyme; ester oil; solvent free; carboligation; esterification;

D O I：

10.1007/s00449-008-0199-3

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The immobilization of an endoglucanase, benzoylformate decarboxylase (BFD) from Pseudomonas putida, as well as of lipase B from Candida antarctica (CALB) onto the carrier supports Sepabeads EC-EP, Sepabeads EC-EA, and Sepabeads EC-BU was accomplished. It is shown that via these immobilized biocatalysts the synthesis of both fine and bulk chemicals is possible. This is illustrated by the syntheses of polyglycerol esters and (S)-hydroxy phenyl propanone. The benefit of immobilization is illustrated by repetitive use in a bubble column reactor as well as in a stirred tank reactor. High stability of two biocatalysts was achieved and reusability up to eight times was demonstrated. The comparison of CALB immobilized on Sepabeads EC-EP to Novozym 435 shows similar activity.

引用

页码：163 / 171

页数：9

共 36 条

[1] Immobilization and stabilization of glutaryl acylase on aminated sepabeads supports by the glutaraldehyde crosslinking method [J].