Structural heterogeneity in DNA: Temperature dependence of 2-aminopurine fluorescence in dinucleotides

被引：41

作者：

Somsen, OJG ^{[1
]}

Keukens, LB ^{[1
]}

de Keijzer, MN ^{[1
]}

van Hoek, A ^{[1
]}

van Amerongen, H ^{[1
]}

机构：

[1] Univ Wageningen & Res Ctr, Biophys Lab, NL-6700 ET Wageningen, Netherlands

来源：

CHEMPHYSCHEM | 2005年 / 6卷 / 08期

关键词：

2-aminopurine; DNA; fluorescence; structural heterogeneity; time-resolved spectroscopy;

D O I：

10.1002/cphc.200400648

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

The fluorescent base analogue 2-aminopurine is a sensitive probe for local dynamics of DNA. Its fluorescence is quenched by interaction with the neighboring bases, but the underlying mechanisms are still under investigation. We studied 2-aminopurine fluorescence in dinucleotides with each of the natural bases. Consistently, two of the four fluorescence-decay components depend strongly on temperature. Our results indicate that these components are due to the excited-state dynamics of a single conformational state. We propose a variation of the gating model in which transient unstacking occurs in the excited state.

引用

页码：1622 / 1627

页数：6

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