Crystallization and preliminary X-ray analysis of porcine muscle prolyl oligopeptidase

被引：6

作者：

Bocskei, Z

Fuxreiter, M

Naray-Szabo, G

Szabo, E

Polgar, L

机构：

[1] Chinoin Chem & Pharmaceut Works Ltd, Dept Chem Res, H-1325 Budapest, Hungary

[2] Eotvos Lorand Univ, Dept Theoret Chem, H-1518 Budapest, Hungary

[3] Agr Biotechnol Ctr, Inst Biochem & Prot Res, H-2101 Godollo, Hungary

[4] Hungarian Acad Sci, Biol Res Ctr, Inst Enzymol, H-1518 Budapest, Hungary

来源：

ACTA CRYSTALLOGRAPHICA SECTION D-STRUCTURAL BIOLOGY | 1998年 / 54卷

关键词：

D O I：

10.1107/S0907444998004181

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Prolyl oligopeptidase from pig muscle has been crystallized in complex with an inhibitor, using PEG 8000 and calcium acetate as precipitants. The crystals are orthorombic and the space group is P2(1)2(1)2(1) With cell dimensions a = 111.8, b = 101.8, c = 72.4 Angstrom. The asymmetric unit contains a single chain of prolyl oligopeptidase,corresponding to a specific volume of 2.55 Angstrom(3) Da(-1) and solvent-content of 52%. The observed diffraction pattern extends to 2.3 Angstrom resolution and the native crystals are well suited for structural analysis by X-ray diffraction methods.

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页码：1414 / 1415

页数：2

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