Formation of a new receptor-operated channel by heteromeric assembly of TRPP2 and TRPC1 subunits

被引:131
作者
Bai, Chang-Xi [1 ]
Giamarchi, Aurelie [2 ]
Rodat-Despoix, Lise [2 ]
Padilla, Francoise [2 ]
Downs, Tamyra [1 ]
Tsiokas, Leonidas [1 ]
Delmas, Patrick [2 ]
机构
[1] Univ Oklahoma, Hlth Sci Ctr, Oklahoma City, OK 73104 USA
[2] Univ Aix Marseille 2, IFR Jean Roche, CNRS, UMR 6231, F-13916 Marseille 20, France
关键词
TRP channel; polycystic kidney disease; mechanosensation; primary cilium; polycystin; 2; 1;
D O I
10.1038/embor.2008.29
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Although several protein-protein interactions have been reported between transient receptor potential (TRP) channels, they are all known to occur exclusively between members of the same group. The only intergroup interaction described so far is that of TRPP2 and TRPC1; however, the significance of this interaction is unknown. Here, we show that TRPP2 and TRPC1 assemble to form a channel with a unique constellation of new and TRPP2/TRPC1-specific properties. TRPP2/TRPC1 is activated in response to G-protein-coupled receptor activation and shows a pattern of single-channel conductance, amiloride sensitivity and ion permeability distinct from that of TRPP2 or TRPC1 alone. Native TRPP2/TRPC1 activity is shown in kidney cells by complementary gain-of-function and loss-of-function experiments, and its existence under physiological conditions is supported by colocalization at the primary cilium and by co-immunoprecipitation from kidney membranes. Identification of the heteromultimeric TRPP2/TRPC1 channel has implications in mechanosensation and cilium-based Ca(2+) signalling.
引用
收藏
页码:472 / 479
页数:8
相关论文
共 33 条
[1]   TRPC1: a core component of store-operated calcium channels [J].
Ambudkar, I. S. .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2007, 35 :96-100
[2]   TRPC1: store-operated channel and more [J].
Beech, DJ .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2005, 451 (01) :53-60
[3]   G protein-coupled receptors sense fluid shear stress in endothelial cells [J].
Chachisvilis, Mirianas ;
Zhang, Yan-Liang ;
Frangos, John A. .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (42) :15463-15468
[4]   Constitutive activation of G-proteins by polycystin-1 is antagonized by polycystin-2 [J].
Delmas, P ;
Nomura, H ;
Li, XG ;
Lakkis, M ;
Luo, Y ;
Segal, Y ;
Fernández-Fernández, JM ;
Harris, P ;
Frischauf, AM ;
Brown, DA ;
Zhou, J .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (13) :11276-11283
[5]   Gating of the polycystin ion channel signaling complex in neurons and kidney cells [J].
Delmas, P ;
Nauli, SM ;
Li, XG ;
Coste, B ;
Osorio, N ;
Crest, M ;
Brown, DA ;
Zhou, J .
FASEB JOURNAL, 2004, 18 (02) :740-+
[6]   Polycystins: polymodal receptor/ion-channel cellular sensors [J].
Delmas, P .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2005, 451 (01) :264-276
[7]   Polycystins: From mechanosensation to gene regulation [J].
Delmas, P .
CELL, 2004, 118 (02) :145-148
[8]   Signaling microdomains define the specificity of receptor-mediated InsP3 pathways in neurons [J].
Delmas, P ;
Wanaverbecq, N ;
Abogadie, FC ;
Mistry, M ;
Brown, DA .
NEURON, 2002, 34 (02) :209-220
[9]   Pressure-induced and store-operated cation influx in vascular smooth muscle cells is independent of TRPC1 [J].
Dietrich, Alexander ;
Kalwa, Hermann ;
Storch, Ursula ;
Mederos y Schnitzler, Michael ;
Salanova, Birgit ;
Pinkenburg, Olaf ;
Dubrovska, Galyna ;
Essin, Kirill ;
Gollasch, Maik ;
Birnbaumer, Lutz ;
Gudermann, Thomas .
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 2007, 455 (03) :465-477
[10]   The versatile nature of the calcium-permeable cation channel TRPP2 [J].
Giamarchi, Aurelie ;
Padilla, Francoise ;
Coste, Bertrand ;
Raoux, Matthieu ;
Crest, Marcel ;
Honore, Eric ;
Delmas, Patrick .
EMBO REPORTS, 2006, 7 (08) :787-793