The effect of leptin on feeding-regulating neurons in the rat hypothalamus

被引：53

作者：

Funahashi, H

Yada, T

Muroya, S

Takigawa, M

Ryushi, T

Horie, S

Nakai, Y

Shioda, S ^{[1
]}

机构：

[1] Showa Univ, Sch Med, Dept Anat, Tokyo 1428555, Japan

[2] Kagoshima Univ, Sch Med, Dept Physiol, Kagoshima 8900075, Japan

[3] Inst Physiol Sci, Lab Intracellular Metab, Okazaki, Aichi 4448585, Japan

[4] Kagoshima Univ, Sch Med, Dept Psychiat, Kagoshima 8900075, Japan

[5] Tokyo Metropolitan Univ, Grad Sch Sci, Lab Human Physiol, Tokyo 1920397, Japan

[6] Tokai Univ, Sch Phys Educ, Dept Phys Recreat, Kanagawa 2591292, Japan

来源：

NEUROSCIENCE LETTERS | 1999年 / 264卷 / 1-3期

关键词：

leptin; leptin receptor; immunohistochemistry; cytosolic Ca2+; rat hypothalamus; glucose-sensitive neuron; glucose-responsive neuron;

D O I：

10.1016/S0304-3940(99)00186-X

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Intense immunoreactivity for the leptin receptor was detected in the hypothalamic arcuate nucleus (ARC), ventromedial nucleus (VMH), and lateral hypothalamus (LH) by immunohistochemistry. Cytosolic C2+ concentration ([Ca2+](i)) in single neurons isolated from the ARC, VMH and LH was measured with dual wavelength fura-2 fluorescence imaging. A reduction of the superfusate glucose concentration from 10 to 1 mM increased [Ca2+](i) in 21% of ARC neurons and 22% of LH neurons. Leptin at 0.1 nM inhibited the [Ca2+](i) increase in 66 and 64% of these glucose-sensitive ARC and LH neurons, respectively. Inversely, 10 mM glucose increased [Ca2+](i) in 49% of the VMH neurons, and 0.1 nM leptin at 1 mM glucose also increased [Ca2+](i) in 84% of these glucose-responsive neurons. These results reveal that leptin inhibits the ARC and LH neurons and stimulates the VMH neurons via the leptin receptor expressed in these cells. (C) 1999 Elsevier Science Ireland Ltd. Ail rights reserved.

引用

页码：117 / 120

页数：4

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