A novel hepatic stellate (Ito) cell-derived protein, epimorphin, plays a key role in the late stages of liver regeneration

被引：37

作者：

Watanabe, S ^{[1
]}

Hirose, M ^{[1
]}

Wang, XE ^{[1
]}

Ikejima, K ^{[1
]}

Oide, H ^{[1
]}

Kitamura, T ^{[1
]}

Takei, Y ^{[1
]}

Miyazaki, A ^{[1
]}

Sato, N ^{[1
]}

机构：

[1] Juntendo Univ, Sch Med, Dept Gastroenterol, Bunkyo Ku, Tokyo 1138421, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 250卷 / 02期

关键词：

D O I：

10.1006/bbrc.1998.9339

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Limited data exist regarding morphogenesis and differentiation during liver regeneration. We examined the role of epimorphin on liver regeneration, After 70% partial hepatectomy, mouse liver was collected on days 1, 3, 7, and 14 for immunohistochemistry and the detection of epimorphin mRNA and connexin 32. Using primary cultured rat hepatocytes, morphogenesis and differentiation of cells were tested with or without epimorphin. Seven days after cell inoculation, the expression of connexin 32 and the cell-cell communication was tested as a marker of differentiation. Epimorphin was detected exclusively in hepatic stellate cells. Connexin 32 was detected only in hepatocytes. After partial hepatectomy, epimorphin mRNA was detected on day 3 and peaked at day 7, followed by protein expression. Connexin 32 expression showed a similar time course. Cultured hepatocytes formed multicellular spheroids in an active epimorphin-coated culture dish and showed positive dye coupling, whereas the cell-cell communication was lost without active epimorphin. Because epimorphin was expressed late in liver regeneration, it might play a role in morphogenesis and differentiation. (C) 1998 Academic Press.

引用

页码：486 / 490

页数：5

共 26 条

[11] New function of Ito cells in liver morphogenesis: Evidence using a novel morphogenic protein, epimorphin, in vitro [J].