Amperometric PMMA-microchip with integrated gold working electrode for enzyme assays

被引：13

作者：

Castaño-Alvarez, M ^{[1
]}

Fernández-Abedul, MT ^{[1
]}

Costa-García, A ^{[1
]}

机构：

[1] Univ Oviedo, Dept Quim Fis & Analit, E-33006 Oviedo, Asturias, Spain

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2005年 / 382卷 / 02期

关键词：

microchip; electrochemical detection; in-channel; gold film; enzyme assay;

D O I：

10.1007/s00216-005-3151-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The use of a gold film in-channel detector combined with a poly( methyl methacrylate) ( PMMA) CE microchip has been tested for alkaline phosphatase (AP) enzymatic assays. Tris-borate or Tris-Gly (pH 9.0, 50 mmol L-1) buffer solutions were appropriate as running buffer. Signals for three common AP products: alpha- naphthol, p-nitrophenol, and ascorbic acid, were obtained. They were reproducible (RSD 4.4% for six successive electropherograms corresponding to 5 mmol L-1 a- naphthol solution) and the response was dependent on concentration ( linear relationship for ascorbic acid solutions between 5 and 20 mmol L-1 concentration). Use of an end- channel gold. lm electrode was also investigated. If one of the reagents (substrate or enzyme) is included in the running buffer, two different types of enzymatic assay are feasible in less than 3 min.

引用

页码：303 / 310

页数：8

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