Role of adhesion molecules in natural killer cell-induced DNA fragmentation of cytomegalovirus-infected fibroblasts

被引：4

作者：

Ito, M ^{[1
]}

Watanabe, M ^{[1
]}

Kamiya, H ^{[1
]}

Sakurai, M ^{[1
]}

机构：

[1] MIE NATL HOSP, DEPT PEDIAT, TSU, MIE, JAPAN

来源：

VIRAL IMMUNOLOGY | 1996年 / 9卷 / 04期

关键词：

D O I：

10.1089/vim.1996.9.219

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

We investigated the roles of CD11a, CD11b, CD18, and CD54 adhesion molecules in nonadherent peripheral blood mononuclear cells (NPBMC)-induced DNA fragmentation of cytomegalovirus (CMV)-infected cells. DNA fragmentation in the supernatant from CMV-infected cells and NPBMC was assayed, and cytotoxicity against CMV-infected cells was calculated. Treatment of NPBMC with monoclonal antibodies to CD11a, CD11b, CD18, and CD54 significantly reduced cytotoxicity against CMV-infected cells. A combination of anti-CD11a, anti-CD11b, and anti-CD18 antibodies further inhibited cytotoxicity against CMV-infected cells. Cytotoxicity against CMV-infected cells treated with anti-CD54 antibody was also significantly inhibited. Binding of effector cells to target cells was not affected by treatment of NPBMC or CMV-infected cells with antiadhesion molecule antibodies. These results indicate that LFA-1 (CD11a/CD18), Mac-1 (CD11b/CD18), and ICAM-1 (CD54) adhesion molecules are involved in natural killer (NK) cell-induced DNA fragmentation in CMV-infected cells.

引用

页码：219 / 224

页数：6

共 21 条

[1] EXPRESSION OF THE ADHESION MOLECULES ICAM-1 AND ICAM-2 ON TUMOR-CELL LINES DOES NOT CORRELATE WITH THEIR SUSCEPTIBILITY TO NATURAL-KILLER CELL-MEDIATED CYTOLYSIS - EVIDENCE FOR ADDITIONAL LIGANDS FOR EFFECTOR CELL BETA-2 INTEGRINS [J].