Organic phase PPO biosensor based on hydrophilic films of electropolymerized polypyrrole

被引：22

作者：

Cristea, C

Mousty, C

Cosnier, S

Popescu, IC

机构：

[1] Univ Grenoble 1, Inst Chim Mol Grenoble, Lab Electrochim Organ & Photochim Redox, CNRS,UMR 5630,FR 2607, F-938041 Grenoble, France

[2] Univ Med & Pharm Iuliu Hatieganu Cluj Napoca, Fac Pharm, Dept Analyt Chem, RO-400010 Cluj Napoca, Romania

[3] Univ Babes Bolyai, Dept Phys Chem, RO-400028 Cluj Napoca, Romania

来源：

ELECTROCHIMICA ACTA | 2005年 / 50卷 / 18期

关键词：

catechol biosensor; polyphenol oxidase; hydrophilic polypyrrole film;

D O I：

10.1016/j.electacta.2005.01.017

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

We described herein, the construction of an organic phase enzyme electrode (OPEE) via polyphenol oxidase (PPO) entrapment within a hydrophilic polypyrrole film electrogenerated from on a new bispyrrolic derivative (1) containing a long hydrophilic spacer. The so-called "adsorption step procedure" was adopted for the preparation of the organic phase PPO biosensor. The amperometric detection of catechol was carried out in anhydrous chloroform at -0.2 V versus Ag/AgCl. The electroanalytical parameters of the biosensor strongly depend on its configuration and on the hydration state of the enzyme matrix. The best sensitivity obtained for catechol in chloroform was 15.6 mA M-1 cm(-2). (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：3713 / 3718

页数：6

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