Metabonomic analysis of mouse urine by liquid-chromatography-time of flight mass spectrometry (LC-TOFMS): detection of strain, diurnal and gender differences

被引：117

作者：

Plumb, R

Granger, J

Stumpf, C

Wilson, ID ^{[1
]}

Evans, JA

Lenz, EM

机构：

[1] AstraZeneca, Dept Drug Metab & Pharmacokinet, Macclesfield SK10 4TG, Cheshire, England

[2] Waters Corp, Life Sci Res & Dev, Milford, MA 01757 USA

来源：

ANALYST | 2003年 / 128卷 / 07期

关键词：

D O I：

10.1039/b304296k

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of HPLC-MS combined with principal components analysis (PCA) to the metabonomic analysis of mouse urine is demonstrated. Urine samples from three strains of mouse were analysed by gradient HPLC-MS combined with positive and negative electrospray time-of-flight mass spectrometry. Analysis of the resulting data using PCA enabled the samples to be discriminated between on the basis of gender, strain and diurnal variation. These preliminary results suggest that HPLC-MS-based approaches may have a useful role in metabonomic analysis that complements existing approaches.

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收藏

页码：819 / 823

页数：5

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