The binding of seven tRNA anticodons to their complementary codons on Escherichia coli ribosomes was substantially impaired, as compared with the binding of their natural tRNAs, when they were transplanted into tRNA(2)(Ala). An analysis of chimeras composed of tRNA(2)(Ala) and various amounts of either tRNA(3)(Gly) or tRNA(2)(Arg) indicates that the presence of the parental 32-38 nucleotide pair is sufficient to restore ribosome binding of the transplanted anticodons. Furthermore, mutagenesis of tRNA(2)(Ala) showed that its highly conserved A32-U38 pair serves to weaken ribosome affinity. We propose that this negative binding determinant is used to offset the very tight codon-anticodon interaction of tRNA(2)(Ala). This suggests that each tRNA sequence has coevolved with its anticodon to tune ribosome affinity to a value that is the same for all tRNAs.
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Univ Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA
Noller, HF
Hoang, L
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Univ Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA
Hoang, L
Fredrick, K
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Univ Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA
机构:
Univ Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA
Noller, HF
Hoang, L
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机构:
Univ Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA
Hoang, L
Fredrick, K
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Univ Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USAUniv Calif Santa Cruz, Ctr Mol Biol RNA, Dept Mol Cell & Dev Biol, Santa Cruz, CA 95064 USA