Improved selectivity for the binding of naphthyridine dimer to guanine-guanine mismatch

被引:105
作者
Nakatani, K [1 ]
Sando, S [1 ]
Saito, I [1 ]
机构
[1] Kyoto Univ, Fac Engn, Dept Synthet Chem & Biol Chem, CREST, Kyoto 6068501, Japan
关键词
D O I
10.1016/S0968-0896(01)00160-2
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Naphthyridine dimer composed of two 2-amino-1,8-naphthyridines and a connecting linker strongly binds to guanine-guanine (G-G) mismatch in duplex DNA. In order to improve G-G selectivity for the binding, we have examined structure modification of the linker. A new naphthyridine dimer possessing 3,6-diazaoctanedioic acid linker binds to G-G mismatch with an association constant of 1.18 x 10(7) M-1, which is somewhat weaker than that of the original naphthyridine dimer having a shorter connecting linker. However, the binding of the modified naphthyridine dimer to G-A mismatch was almost negligible as compared to that of the original. This results in a net increase of the selectivity for the binding to G-G mismatch by 4-folds. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:2381 / 2385
页数:5
相关论文
共 21 条
[1]   Determination of single-nucleotide polymorphisms by real-time pyrophosphate DNA sequencing [J].
Alderborn, A ;
Kristofferson, A ;
Hammerling, U .
GENOME RESEARCH, 2000, 10 (08) :1249-1258
[2]  
BRENOWITZ M, 1986, METHOD ENZYMOL, V130, P132
[3]   Variations on a theme: Cataloging human DNA sequence variation [J].
Collins, FS ;
Guyer, MS ;
Chakravarti, A .
SCIENCE, 1997, 278 (5343) :1580-1581
[4]   Self-association without regard to prototropy. A heterocycle that forms extremely stable quadruply hydrogen-bonded dimers [J].
Corbin, PS ;
Zimmerman, SC .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1998, 120 (37) :9710-9711
[5]   CURRENT METHODS OF MUTATION DETECTION [J].
COTTON, RGH .
MUTATION RESEARCH, 1993, 285 (01) :125-144
[6]   BIAcore for macromolecular interaction [J].
Fivash, M ;
Towler, EM ;
Fisher, RJ .
CURRENT OPINION IN BIOTECHNOLOGY, 1998, 9 (01) :97-101
[7]   A BISUBSTRATE REACTION TEMPLATE [J].
KELLY, TR ;
ZHAO, C ;
BRIDGER, GJ .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1989, 111 (10) :3744-3745
[8]   Genotyping by mass spectrometric analysis of short DNA fragments [J].
Laken, SJ ;
Jackson, PE ;
Kinzler, KW ;
Vogelstein, B ;
Strickland, PT ;
Groopman, JD ;
Friesen, MD .
NATURE BIOTECHNOLOGY, 1998, 16 (13) :1352-1356
[9]   A general approach to single-nucleotide polymorphism discovery [J].
Marth, GT ;
Korf, I ;
Yandell, MD ;
Yeh, RT ;
Gu, ZJ ;
Zakeri, H ;
Stitziel, NO ;
Hillier, L ;
Kwok, PY ;
Gish, WR .
NATURE GENETICS, 1999, 23 (04) :452-456
[10]   CONVENIENT SYNTHESIS OF BIFUNCTIONAL TETRAAZA MACROCYCLES [J].
MCMURRY, TJ ;
BRECHBIEL, M ;
KUMAR, K ;
GANSOW, OA .
BIOCONJUGATE CHEMISTRY, 1992, 3 (02) :108-117