A Dual Platform for Selective Analyte Enrichment and Ionization in Mass Spectrometry Using Aptamer-Conjugated Graphene Oxide

被引：176

作者：

Gulbakan, Basri ^{[1
,2
]}

Yasun, Emir ^{[1
,2
]}

Shukoor, M. Ibrahim ^{[1
,2
]}

Zhu, Zhi ^{[1
,2
]}

You, Mingxu ^{[1
,2
]}

Tan, Xiaohong ^{[1
,2
,3
]}

Sanchez, Hernan ^{[4
,5
]}

Powell, David H. ^{[1
,2
]}

Dai, Hongjie ^{[4
,5
]}

Tan, Weihong ^{[1
,2
,3
]}

机构：

[1] Univ Florida, Dept Chem, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA

[2] Univ Florida, Dept Physiol & Funct Gen, Shands Canc Ctr, Gainesville, FL 32611 USA

[3] Hunan Univ, State Key Lab Chemo Biosensing & Chemometrics, Coll Biol, Coll Chem & Chem Engn, Changsha 410082, Hunan, Peoples R China

[4] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

[5] Stanford Univ, Adv Mat Lab, Stanford, CA 94305 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2010年 / 132卷 / 49期

关键词：

PLASMA; NANOPARTICLES; CARBON; LONG; MS;

D O I：

10.1021/ja109042w

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

This study demonstrates the use of aptamer-conjugated graphene oxide as an affinity extraction and detection platform for analytes from complex biological media. We have shown that cocaine and adenosine can be selectively enriched from plasma samples and that direct mass spectrometric readouts can be obtained without a matrix and with greatly improved signal-to-noise ratios. Aptamer-conjugated graphene oxide has clear advantages in target enrichment and in generating highly efficient ionization of target molecules for mass spectrometry. These results demonstrate the utility of the approach for analysis of small molecules in real biological samples.

引用

页码：17408 / 17410

页数：3

共 30 条

[1] Honeycomb Carbon: A Review of Graphene [J].

Allen, Matthew J. ;

Tung, Vincent C. ;

Kaner, Richard B. .

CHEMICAL REVIEWS, 2010, 110 (01) :132-145

[2] Affinity purification-mass spectrometry - Powerful tools for the characterization of protein complexes [J].

Bauer, A ;

Kuster, B .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 2003, 270 (04) :570-578

[3] Rapid and Selective Screening for Sulfhydryl Analytes in Plasma and Urine Using Surface-Enhanced Transmission Mode Desorption Electrospray Ionization Mass Spectrometry [J].

Chipuk, Joseph E. ;

Gelb, Michael H. ;

Brodbelt, Jennifer S. .

ANALYTICAL CHEMISTRY, 2010, 82 (10) :4130-4139

[4] Nanoprobe-based affinity mass spectrometry for selected protein profiling in human plasma [J].

Chou, PH ;

Chen, SH ;

Liao, HK ;

Lin, PC ;

Her, GR ;

Lai, ACY ;

Chen, JH ;

Lin, CC ;

Chen, YJ .

ANALYTICAL CHEMISTRY, 2005, 77 (18) :5990-5997

[5] Functional aptamers and aptazymes in biotechnology, diagnostics, and therapy [J].

Famulok, Michael ;

Hartig, Jorg S. ;

Mayer, Gunter .

CHEMICAL REVIEWS, 2007, 107 (09) :3715-3743

[6] Aptamers Generated from Cell-SELEX for Molecular Medicine: A Chemical Biology Approach [J].

Fang, Xiaohong ;

Tan, Weihong .

ACCOUNTS OF CHEMICAL RESEARCH, 2010, 43 (01) :48-57

[7] Centimeter-Long and Large-Scale Micropatterns of Reduced Graphene Oxide Films: Fabrication and Sensing Applications [J].

He, Qiyuan ;

Sudibya, Herry Gunadi ;

Yin, Zongyou ;

Wu, Shixin ;

Li, Hai ;

Boey, Freddy ;

Huang, Wei ;

Chen, Peng ;

Zhang, Hua .

ACS NANO, 2010, 4 (06) :3201-3208

[8] Screening Assay of Very Long Chain Fatty Acids in Human Plasma with Multiwalled Carbon Nanotube-Based Surface-Assisted Laser Desorption/Ionization Mass Spectrometry [J].

Hsu, Wei-Yi ;

Lin, Wei-De ;

Hwu, Wuh-Liang ;

Lai, Chien-Chen ;

Tsai, Fuu-Jen .

ANALYTICAL CHEMISTRY, 2010, 82 (16) :6814-6820

[9] Analysis of adenosine triphosphate and glutathione through gold nanoparticles assisted laser desorption/ionization mass spectrometry [J].

Huang, Yu-Fen ;

Chang, Huan-Tsung .

ANALYTICAL CHEMISTRY, 2007, 79 (13) :4852-4859

[10] NEW DESORPTION STRATEGIES FOR THE MASS-SPECTROMETRIC ANALYSIS OF MACROMOLECULES [J].

HUTCHENS, TW ;

YIP, TT .

RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 1993, 7 (07) :576-580

← 1 2 3 →