L-Theanine Down-Regulates the JAK/STAT3 Pathway to Attenuate the Proliferation and Migration of Vascular Smooth Muscle Cells Induced by Angiotensin II

被引:29
作者
Ben, Peiling [1 ]
Hu, Monong [1 ]
Wu, Huizhen [1 ]
Zhang, Zhengping [2 ]
Gao, Yanhong [3 ]
Luo, Lan [2 ]
Yin, Zhimin [3 ]
机构
[1] Chuzhou City Vocat Coll, Dept Med, Chuzhou 239000, Anhui, Peoples R China
[2] Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210093, Jiangsu, Peoples R China
[3] Nanjing Normal Univ, Coll Life Sci, Jiangsu Prov Key Lab Mol & Med Biotechnol, Nanjing 210023, Jiangsu, Peoples R China
关键词
L-theanine; vascular smooth muscle cell; proliferation; signal transducer and activator of transcription 3 (STAT3); Janus kinase 2 (JAK2); angiotensin II; ACTIVATION; EXPRESSION; PROMOTES; INJURY; RATS; SRC; INHIBITION; TEA;
D O I
10.1248/bpb.b18-00387
中图分类号
R9 [药学];
学科分类号
100702 [药剂学];
摘要
L-Theanine, a green tea amino acid derivative, has cardiovascular qualities. The focus of the current evaluation was to examine the suppression of L-theanine on cultured vascular smooth muscle cell (VSMC) proliferation and migration that is prompted by angiotensin II (Ang H). The VSMCs were treated with noncytotoxic concentrations of L-theanine and then stimulated with Ang II. The CCK-8 and Transwell chamber assays were monitored on the proliferation and migration rate, respectively. We discovered that L-theanine (50 and 100 mu m) significantly halted Ang II-induced VSMC proliferation and migration. This was joined by a decline in the amount of cyclin Dl. An additional discovery was that L-theanine lowered the proportion of S-phase cells, whereas the number of Gl/GO-phase cells in Ang II-stimulated VSMCs was elevated, based on flow cytometry. Western blotting analyses indicated that L-theanine had no impact on extracellularsignal-regulated kinase 1/2 (ERK1/2) activation prompted by Ang II. Nevertheless, L-theanine significantly lowered Ang II-prompted phosphorylation of Janus kinase 2 (JAK2), c-Src tyrosine kinase, and signal transducer and activators of transcription 3 (STAT3). The outcomes revealed that L-theanine subdued the Ang II-prompted proliferation and migration of VSMC, partly via the obstruction of the JAK/STAT3 pathway instead of via just the ERK pathway.
引用
收藏
页码:1678 / 1684
页数:7
相关论文
共 36 条
[1]
Involvement of c-Src Tyrosine Kinase in SHP-1 Phosphatase Activation by Ang II AT2 Receptors in Rat Fetal Tissues [J].
Alvarez, Sergio E. ;
Seguin, Leonardo R. ;
Villarreal, Rodrigo S. ;
Nahmias, Clara ;
Ciuffo, Gladys M. .
JOURNAL OF CELLULAR BIOCHEMISTRY, 2008, 105 (03) :703-711
[2]
Angiotensin II activation of the JAK/STAT pathway in mesangial cells is altered by high glucose [J].
Amiri, F ;
Shaw, S ;
Wang, XD ;
Tang, J ;
Waller, JL ;
Eaton, DC ;
Marrero, MB .
KIDNEY INTERNATIONAL, 2002, 61 (05) :1605-1616
[3]
L-Theanine attenuates cadmium-induced neurotoxicity through the inhibition of oxidative damage and tau hyperphosphorylation [J].
Ben, Peiling ;
Zhang, Zhengping ;
Zhu, Yanyan ;
Xiong, Aiying ;
Gao, Yanhong ;
Mu, Jianyun ;
Yin, Zhimin ;
Luo, Lan .
NEUROTOXICOLOGY, 2016, 57 :95-103
[4]
Control of cell cycle transcription during G1 and S phases [J].
Bertoli, Cosetta ;
Skotheim, Jan M. ;
de Bruin, Robertus A. M. .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2013, 14 (08) :518-528
[5]
A 13-week dietary toxicity and toxicokinetic study with L-theanine in rats [J].
Borzelleca, J. F. ;
Peters, D. ;
Hall, W. .
FOOD AND CHEMICAL TOXICOLOGY, 2006, 44 (07) :1158-1166
[6]
NADPH oxidases and angiotensin II receptor signaling [J].
Garrido, Abel Martin ;
Griendling, Kathy K. .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 2009, 302 (02) :148-158
[7]
ANG II-stimulated DNA synthesis is mediated by ANG II receptor-dependent Ca2+/PKC as well as EGF receptor-dependent PI3K/Akt/mTOR/p70S6K1 signal pathways in mouse embryonic stem cells [J].
Han, Ho Jae ;
Han, Ji Yeon ;
Heo, Jung Sun ;
Lee, Sang Hun ;
Lee, Min Young ;
Kim, Yun Hee .
JOURNAL OF CELLULAR PHYSIOLOGY, 2007, 211 (03) :618-629
[8]
Angiotensin-converting enzyme and vascular remodeling [J].
Heeneman, Sylvia ;
Sluimer, Judith C. ;
Daemen, Mat J. A. P. .
CIRCULATION RESEARCH, 2007, 101 (05) :441-454
[9]
Hou L, 2010, CHINESE J HYPERTENSI, V23, P701
[10]
Vascular smooth muscle Jak2 deletion prevents angiotensin II-mediated neointima formation following injury in mice [J].
Kirabo, Annet ;
Oh, S. Paul ;
Kasahara, Hideko ;
Wagner, Kay-Uwe ;
Sayeski, Peter P. .
JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY, 2011, 50 (06) :1026-1034