Leptin receptor signal transduction:: OBRa and OBRb of fa type

被引：133

作者：

Yamashita, T

Murakami, T

Otani, S

Kuwajima, M

Shima, K

机构：

[1] Univ Tokushima, Sch Med, Dept Lab Med, Tokushima 7708503, Japan

[2] Otsuka Pharmaceut Co Ltd, Tokushima Inst 2, Tokushima 7710192, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 246卷 / 03期

关键词：

D O I：

10.1006/bbrc.1998.8689

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report herein the characterization of activities of signal transduction for three types of leptin receptors (OBRs) from rats, the OBRa, OBRb, and OBRb with fa mutation (OBRb-fa), by measurement of the levels of tyrosine phosphorylation of STAT3 (signal transducers and activators of transcription 3) and MAPK (mitogen-activated protein kinase), which are induced by leptin stimulation of CHO cells stably expressing the OBR (CHO-OBRb, CHO-OBRa, or CHO-OBRb-fa cells). As the result of leptin stimulation, enhanced levels of tyrosine phosphorylation of STAT3 and MAPK were detected in CHO-OBRb cells. In CHO-OBRb-fa cells, enhancement levels for both were lower than those in CHO-OBRb cells. In CHO-OBRa cells, only the phosphorylation of MAPK was detected. These data suggest that these reduced signaling activities cause obesity in fa/fa rats and that OBRa, which has been generally thought to be inactive at signaling, actually transmits signals through the MAPK pathway. (C) 1998 Academic Press.

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页码：752 / 759

页数：8

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