Change in secondary structure of silk fibroin during preparation of its microspheres by spray-drying and exposure to humid atmosphere

被引：109

作者：

Hino, T ^{[1
]}

Tanimoto, M ^{[1
]}

Shimabayashi, S ^{[1
]}

机构：

[1] Univ Tokushima, Fac Pharmaceut Sci, Tokushima 7708505, Japan

来源：

JOURNAL OF COLLOID AND INTERFACE SCIENCE | 2003年 / 266卷 / 01期

关键词：

silk fibroin; secondary structure; spray-drying; water uptake; beta-sheet; irregular structure;

D O I：

10.1016/S0021-9797(03)00584-8

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Silk microspheres prepared by spray-drying were exposed to humid atmosphere (89% relative humidity, RH). Change in the secondary structure of silk fibroin during preparation of silk microspheres and exposure to high humidity was studied. Scoured silk fiber was dissolved in an aqueous solution of calcium chloride mixed with ethanol. After dialysis against purified water, theophylline was added to the solution as a model drug. Silk microspheres were obtained by spray-drying. Silk fibroin and theophylline were found to be amorphous in the microsphere by means of powder X-ray diffractometry. Fibroin assumed beta-sheet conformation in the scoured silk fiber while it has an irregular structure in the microsphere, according to FTIR and Raman spectra. Fibroin recrystallized and its secondary structure changed to beta-sheet conformation by exposure of the microspheres to an atmosphere of 89% RH. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：68 / 73

页数：6

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