Preparation and functional characterization of thylakoids from Arabidopsis thaliana

被引：70

作者：

Casazza, AP ^{[1
]}

Tarantino, D ^{[1
]}

Soave, C ^{[1
]}

机构：

[1] Univ Milan, Dept Biol, I-20133 Milan, Italy

来源：

PHOTOSYNTHESIS RESEARCH | 2001年 / 68卷 / 02期

关键词：

D O I：

10.1023/A:1011818021875

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

A protocol for the isolation of functional thylakoids from Arabidopsis thaliana leaves was developed. The critical factor in obtaining active, coupled and stable preparation is the inclusion of EDTA and EGTA in the grinding buffer. Preparations were characterized with respect to the whole or partial electron transport chain, ATP/NADPH, ATP/O-2 and PS II/chlorophyll ratios. Sensitivity to a light-chill photoinhibitory treatment was also determined by evaluating the decrease in both maximal photochemical efficiency (Fv/Fm) and in electron transport rate.

引用

页码：175 / 180

页数：6

共 19 条

[11] THE EFFECTS OF REDUCED AMOUNTS OF LIPID UNSATURATION ON CHLOROPLAST ULTRASTRUCTURE AND PHOTOSYNTHESIS IN A MUTANT OF ARABIDOPSIS [J].