B Cells With High Side Scatter Parameter by Flow Cytometry Correlate With Inferior Survival in Diffuse Large B-Cell Lymphoma

被引：7

作者：

Bashashati, Ali ^{[2
]}

Johnson, Nathalie A. ^{[6
]}

Khodabakhshi, Alireza Hadj ^{[4
]}

Whiteside, Matthew D. ^{[7
]}

Zare, Habil ^{[1
]}

Scott, David W. ^{[3
]}

Lo, Kenneth ^{[8
]}

Gottardo, Raphael ^{[9
]}

Brinkman, Fiona S. L. ^{[7
]}

Connors, Joseph M. ^{[5
]}

Slack, Graham W. ^{[3
]}

Gascoyne, Randy D. ^{[3
]}

Weng, Andrew P. ^{[1
,3
]}

Brinkman, Ryan R. ^{[1
,10
]}

机构：

[1] British Columbia Canc Agcy, Terry Fox Lab, Vancouver, BC V5Z 1L3, Canada

[2] British Columbia Canc Agcy, Dept Mol Oncol, Vancouver, BC V5Z 1L3, Canada

[3] British Columbia Canc Agcy, Dept Pathol & Lab Med, Vancouver, BC V5Z 1L3, Canada

[4] British Columbia Canc Agcy, Michael Smith Genome Sci Ctr, Vancouver, BC V5Z 1L3, Canada

[5] British Columbia Canc Agcy, Ctr Lymphoid Canc, Vancouver, BC V5Z 1L3, Canada

[6] McGill Univ, Dept Med, Montreal, PQ, Canada

[7] Simon Fraser Univ, Dept Mol Biol & Biochem, Burnaby, BC V5A 1S6, Canada

[8] Univ Washington, Dept Microbiol, Seattle, WA 98195 USA

[9] Fred Hutchinson Canc Res Ctr, Vaccine & Infect Dis Div, Seattle, WA 98104 USA

[10] Univ British Columbia, Dept Med Genet, Vancouver, BC, Canada

来源：

AMERICAN JOURNAL OF CLINICAL PATHOLOGY | 2012年 / 137卷 / 05期

基金：

美国国家卫生研究院; 加拿大自然科学与工程研究理事会; 加拿大健康研究院;

关键词：

Side scatter; Flow cytometry; Diffuse large B-cell lymphoma; Lymphoma; Survival; PROGNOSTIC-SIGNIFICANCE; PROTEIN EXPRESSION; R-CHOP; GENE; BCL-6; CLASSIFICATION; BIOCONDUCTOR;

D O I：

10.1309/AJCPGR8BG4JDVOWR

中图分类号：

R36 [病理学];

学科分类号：

100104 ;

摘要：

Despite advances in the understanding of diffuse large B-cell lymphoma (DLBCL) biology, only the clinically based International Prognostic Index (IPI) is used routinely for risk stratification at diagnosis. To find novel prognostic markers, we analyzed flow cytometric data from 229 diagnostic DLBCL samples using an automated multiparameter data analysis approach developed in our laboratory. By using the developed automated data analysis pipeline, we identified 71 of 229 cases as having more than 35% B cells with a high side scatter (SSC) profile, a parameter reflecting internal cellular complexity. This high SSC B-cell feature was associated with inferior overall and progression-free survival (P = .001 and P = .01, respectively) and remained a significant predictor of overall survival in multivariate Cox regression analysis (IPI, P = .001; high SSC, P = .004; rituximab, P = .53). This study suggests that high SSC among B cells may serve as a useful biomarker to identify patients with DLBCL at high risk for relapse. This is of particular interest because this biomarker is readily available in most clinical laboratories without significant alteration to existing routine diagnostic strategies or incurring additional costs.

引用

页码：805 / 814

页数：10

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