Platelet adhesion on the gradient surfaces grafted with phospholipid polymer

被引:52
作者
Iwasaki, Y
Ishihara, K
Nakabayashi, N
Khang, G
Jeon, JH
Lee, JW
Lee, HB
机构
[1] Tokyo Med & Dent Univ, Inst Med & Dent Engn, Chiyoda Ku, Tokyo 1010062, Japan
[2] Korea Res Inst Chem Technol, Biomat Lab, Taejon 305600, South Korea
关键词
gradient surface; corona irradiation; graft polymerization; phospholipid polymer; protein adsorption; platelet adhesion; blood compatibility;
D O I
10.1163/156856298X00163
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We have synthesized omega-methacryloyloxyalkyl phosphorylcholine (MAPC) polymers as new blood-compatible materials, with attention to the surface structure of the biomembrane and investigated their blood compatibility. The blood compatibility observed on the MAPC polymers is due to their strong affinity to phospholipids. When the blood comes in contact with the MAPC polymer, phospholipids in the plasma preferentially adsorb on the surface, compared with the plasma proteins or cells. The adsorbed phospholipids construct a biomembrane-like structure on the MAPC polymer surface. The MAPC polymers then have an excellent blood compatibility. In this study, we prepared a gradient poly(MAPC)-grafted polyethylene (PE) surface using a corona discharge treatment method to clarify the effect of the chemical structure of the MAPC unit on the blood compatibility of the MAPC polymers. The surface composition of MAPC and the hydrophilicity on the poly(MAPC)-grafted PE surface were determined by X-ray photoelectron spectroscopic (XPS) analysis and contact angle measurement with water, respectively. The phosphorus/carbon (P/C) ratio determined by the XPS analysis increased, but the water contact angle decreased with increasing corona irradiation energy. These results indicated that the surface density of the MAPC unit was increased. More than 2.5 cm from the starting point of;he corona irradiation, the P/C ratio and water contact angle of the surface achieved a constant level. Thus, the surface was completely covered with the grafted poly(MAPC) chain. The effect of the methylene chain length of the MAPC unit on surface properties was also observed. The phospholipid polar group of the MAPC unit was effectively exposed on the surface as the chain length became longer. Moreover, the hydrophobicity of the surface was increased with the increase in the methylene chain length of the MAPC unit. The number of platelets adhering to the poly(MAPC)-grafted PE surface was reduced from the same point where the P/C ratio became constant.
引用
收藏
页码:801 / 816
页数:16
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