Use of magnetic nanobeads to study intracellular antigen processing

被引：9

作者：

Perrin-Cocon, LA

Chesne, S

Pignot-Paintrand, I

Marche, PN

Villiers, CL

机构：

[1] Univ Grenoble 1, CEA Grenoble, DBMS,INSERM U238, Lab Immunochim, F-38054 Grenoble 9, France

[2] CEA, IFR INSERM 27, Dept Biol Mol & Struct, Grenoble, France

来源：

JOURNAL OF MAGNETISM AND MAGNETIC MATERIALS | 2001年 / 225卷 / 1-2期

关键词：

magnetic fractionation; endocytosis; intracellular compartment; B-cell receptor; 2D-electrophoresis; electron microscopy; tetanus toxin; antigen processing; fluorescence microscopy;

D O I：

10.1016/S0304-8853(00)01246-4

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Magnetic nanobeads were covalently linked to antigens and used as a tool to simultaneously follow their intracellular transport into the cells and specifically purify the intracellular compartments implicated in antigen processing. The protein content of these vesicles was analysed by 2D-electrophoresis. Furthermore, nanobeads allowed intracellular localisation of the antigen in electron and fluorescence microscopy. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：161 / 168

页数：8

共 13 条

[1] Type II and III receptors for immunoglobulin G (IgG) control the presentation of different T cell epitopes from single IgG-complexed antigens [J].