Expression of vascular endothelial growth factor and its receptors in rat neural stem cells

被引：122

作者：

Maurer, MH ^{[1
]}

Tripps, WKC ^{[1
]}

Feldmann, RE ^{[1
]}

Kuschinsky, W ^{[1
]}

机构：

[1] Heidelberg Univ, Dept Physiol & Pathophysiol, D-69120 Heidelberg, Germany

来源：

NEUROSCIENCE LETTERS | 2003年 / 344卷 / 03期

关键词：

vascular endothelial growth factor; neural stem cell; angiogenesis; neurogenesis; cell culture; anoxia; rat; HIPPOCAMPAL-NEURONS; FACTOR VEGF; IN-VITRO; MECHANISMS; ANGIOGENESIS; NEUROGENESIS; ISCHEMIA; INJURY; BRAIN;

D O I：

10.1016/S0304-3940(03)00407-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Neural stem cells serve neurogenesis in several areas of the adult mammalian brain. The present study investigates an additional feature, i.e. the expression of vascular endothelial growth factor (VEGF) and its receptors in cultured neurospheres from hippocampus, subventricular zone, and olfactory bulb in adult rats. RT-PCR showed that all three lines expressed VEGF mRNA, but different sets of VEGF receptor mRNAs. Stimulation by either 50 ng/ml VEGF-A165 or by 24 It of anoxia resulted in an altered pattern of receptor expression for VEGF-R2 (Flk-1), VEGF-R3 (Flt-4), neuropilin-1, and neuropilin-2, whereas VEGF-R1 (Flt-1) remained unexpressed. The basal expression of VEGF and of several of its receptor mRNAs indicates a hitherto unknown angiogenic potential of neural stem cells. (C) 2003 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：165 / 168

页数：4

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