Escherichia coli β-galactosidase is heterogeneous with respect to the activity of individual molecules

被引：30

作者：

Craig, DB ^{[1
]}

Dovichi, NJ ^{[1
]}

机构：

[1] Univ Alberta, Dept Chem, Edmonton, AB T6G 2G2, Canada

来源：

CANADIAN JOURNAL OF CHEMISTRY-REVUE CANADIENNE DE CHIMIE | 1998年 / 76卷 / 05期

关键词：

single-molecule chemistry; beta-galactosidase; capillary electrophoresis; laser-induced fluorescence; enzyme assay;

D O I：

10.1139/cjc-76-5-623

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Escherichia coli beta-galactosidase molecules were incubated with fluorogenic substrate in a capillary. Upon flushing the reactor contents past the detector, individual randomly distributed peaks of product were observed, each representing the activity of an individual enzyme molecule. Individual molecules were found to differ with respect to their activities. Molecules showed a 23-fold distribution of activities with the majority of molecules within a 4-fold distribution.

引用

页码：623 / 626

页数：4

共 4 条

[1] FLUORESCENCE-BASED ENZYMATIC ASSAY BY CAPILLARY ELECTROPHORESIS LASER-INDUCED FLUORESCENCE DETECTION FOR THE DETERMINATION OF A FEW BETA-GALACTOSIDASE MOLECULES [J].