Efficient transformation of Medicago truncatula cv. Jemalong using the hypervirulent Agrobacterium tumefaciens strain AGL1

被引：63

作者：

Chabaud, M ^{[1
]}

de Carvalho-Niebel, F ^{[1
]}

Barker, DG ^{[1
]}

机构：

[1] INRA, CNRS, Lab Interact Plantes Micoorganismes, UMR215, F-31326 Castanet Tolosan, France

来源：

PLANT CELL REPORTS | 2003年 / 22卷 / 01期

关键词：

transformation; regeneration; Jemalong; AGL1; Agrobacterium; MEDIATED GENE-TRANSFER; T-DNA; PLANTS; EXPRESSION; REGENERATION; PROMOTER; VECTORS; GAERTN; RI;

D O I：

10.1007/s00299-003-0649-y

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

The efficiency of Agrobacterium tumefaciens transformation of the model legume Medicago truncatula cv. Jemalong (genotype 2HA) was evaluated for strains LBA 4404, C58pMP90, C58pGV2260 and AGL1. Binary vectors carrying promoter-gus/gfp reporter gene fusions and the nptII gene as selectable marker were used for plant in vitro transformation/regeneration. The highest transformation efficiency was obtained with the disarmed hypervirulent strain AGL1 (Ti plasmid TiBo542), for which the percentage of explants forming kanamycin (Km)-resistant calli was double that obtained with each of the other three strains. In addition, we were able to reduce the time necessary for plant regeneration using AGL1, with 24% of the explants generating Km-resistant transgenic plantlets within only 4-5 months of culture. Transgene expression in planta was analysed and found to be conserved in the T-1 descendents.

引用

页码：46 / 51

页数：6

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