Probiotics reduce enterohemorrhagic Escherichia coli O157:H7-and enteropathogenic E-coli O127:H6-induced changes in polarized T84 epithelial cell monolayers by reducing bacterial adhesion and cytoskeletal rearrangements

被引:177
作者
Sherman, PM
Johnson-Henry, KC
Yeung, HP
Ngo, PSC
Goulet, J
Tompkins, TA
机构
[1] Univ Toronto, Hosp Sick Children, Res Inst, Toronto, ON M5G 1X8, Canada
[2] Univ Laval, Laval, PQ, Canada
[3] Inst Rosell Lallemand Inc, Montreal, PQ, Canada
关键词
D O I
10.1128/IAI.73.8.5183-5188.2005
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The aim of this study was to determine if probiotics reduce epithelial injury following exposure to Escherichia coli O157:H7 and E. coli O127:H6. The pretreatment of intestinal (T84) cells with lactic acid-producing bacteria reduced the pathogen-induced drop in transepithelial electrical resistance. These findings demonstrate that probiotics prevent epithelial injury induced by attaching-effacing bacteria.
引用
收藏
页码:5183 / 5188
页数:6
相关论文
共 36 条
[1]   Probiotic bifidobacteria protect mice from lethal infection with shiga toxin-producing Escherichia coli O157:H7 [J].
Asahara, T ;
Shimizu, K ;
Nomoto, K ;
Hamabata, T ;
Ozawa, A ;
Takeda, Y .
INFECTION AND IMMUNITY, 2004, 72 (04) :2240-2247
[2]   Intestinal epithelial responses to enteric pathogens: effects on the tight junction barrier, ion transport, and inflammation [J].
Berkes, J ;
Viswanathan, VK ;
Savkovic, SD ;
Hecht, G .
GUT, 2003, 52 (03) :439-451
[3]   The human Lactobacillus acidophilus strain LA1 secretes a nonbacteriocin antibacterial substance(s) active in vitro and in vivo [J].
BernetCamard, MF ;
Lievin, V ;
Brassart, D ;
Neeser, JR ;
Servin, AL ;
Hudault, S .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (07) :2747-2753
[4]   Inhibitory effect of probiotic Escherichia coli strain Nissle 1917 on adhesion to and invasion of intestinal epithelial cells by adherent-invasive E-coli strains isolated from patients with Crohn's disease [J].
Boudeau, J ;
Glasser, AL ;
Julien, S ;
Colombel, JF ;
Darfeuille-Michaud, A .
ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2003, 18 (01) :45-56
[5]   Invasive ability of an Escherichia coli strain isolated from the ileal mucosa of a patient with Crohn's disease [J].
Boudeau, J ;
Glasser, AL ;
Masseret, E ;
Joly, B ;
Darfeuille-Michaud, A .
INFECTION AND IMMUNITY, 1999, 67 (09) :4499-4509
[6]   Antagonistic action of cells of Lactobacillus lactis toward Escherichia coli O157:H7 on refrigerated raw chicken meat [J].
Brashears, MM ;
Reilly, SS ;
Gilliland, SE .
JOURNAL OF FOOD PROTECTION, 1998, 61 (02) :166-170
[7]   Saccharomyces boulardii preserves the barrier function and modulates the signal transduction pathway induced in enteropathogenic Escherichia coli-infected T84 cells [J].
Czerucka, D ;
Dahan, S ;
Mograbi, B ;
Rossi, B ;
Rampal, P .
INFECTION AND IMMUNITY, 2000, 68 (10) :5998-6004
[8]   Saccharomyces boulardii interferes with enterohemorrhagic Escherichia coli-induced signaling pathways in T84 cells [J].
Dahan, S ;
Dalmasso, G ;
Imbert, V ;
Peyron, JF ;
Rampal, P ;
Czerucka, D .
INFECTION AND IMMUNITY, 2003, 71 (02) :766-773
[9]  
Gedek BR, 1999, MYCOSES, V42, P261, DOI 10.1046/j.1439-0507.1999.00449.x
[10]   Effect of Lactobacillus ingestion on the gastrointestinal mucosal barrier alterations induced by indometacin in humans [J].
Gotteland, M ;
Cruchet, S ;
Verbeke, S .
ALIMENTARY PHARMACOLOGY & THERAPEUTICS, 2001, 15 (01) :11-17