An efficient and versatile solid-phase synthesis of 5′ and 3′-conjugated oligonucleotides

被引：23

作者：

D'Onofrio, J ^{[1
]}

Montesarchio, D ^{[1
]}

De Napoli, L ^{[1
]}

Di Fabio, G ^{[1
]}

机构：

[1] Univ Naples Federico II, Dipartimento Chim Organ & Biochim, I-80126 Naples, Italy

来源：

ORGANIC LETTERS | 2005年 / 7卷 / 22期

关键词：

D O I：

10.1021/ol051811g

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

[GRAPHICS] An easy and efficient solid-phase strategy to obtain 5'- and T-oligonucleotide conjugates in highly pure form has been developed. Ad hoc derivatized solid supports, to which the first nucleoside unit can be attached through a phosphate linkage, have been exploited both in a pre- and post-DNA assembly conjugation approach. A number of 5'- or 3'-oligonucleotide conjugates, incorporating a variety of labels covalently linked through a phosphodiester or a phosphoramidate bond, have been synthesized and characterized.

引用

页码：4927 / 4930

页数：4

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