Synthesis of oleic acid esters catalyzed by immobilized lipase

被引：66

作者：

Habulin, M ^{[1
]}

Krmelj, V ^{[1
]}

Knez, Z ^{[1
]}

机构：

[1] FAC CHEM & CHEM ENGN,MARIBOR 62000,SLOVENIA

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 1996年 / 44卷 / 01期

关键词：

esterification; batch reactor; lipase; Mucor miehei; oleic acid esters; high pressure; carbon dioxide; enzyme stability;

D O I：

10.1021/jf950163k

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Lipase-catalyzed syntheses of oleic acid esters with various primary alcohols have been performed in a batch stirred tank reactor in an almost nonaqueous medium without organic solvents. For all syntheses 50 degrees C was found to be the optimal temperature. Initial reaction rates were influenced by the alcohol chain length. The study of the pressure stability of the immobilized lipase from Rhizomucor miehei (lipozyme IM) showed that the lipase preparation is quite stable; it does not lose its activity when it is exposed to carbon dioxide at 300 bar for 24 h. Esterification rates at high pressure were determined, and it was found that they were higher than at atmospheric pressure. The highest rate and maximal conversion were near the critical point of carbon dioxide.

引用

页码：338 / 342

页数：5