Analysis of α-linolenic acid biohydrogenation intermediates in milk fat with emphasis on conjugated linolenic acids

被引:121
作者
Destaillats, F
Trottier, JP
Galvez, JMG
Angers, P [1 ]
机构
[1] Univ Laval, Dept Food Sci & Nutr, Ste Foy, PQ G1K 7P4, Canada
[2] Univ Laval, Dairy Res Ctr STELA, Ste Foy, PQ G1K 7P4, Canada
[3] Naturia Inc, Sherbrooke, PQ J1L 2P4, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
biohydrogenation; conjugated linolenic acid; milk fat; rumelenic acid;
D O I
10.3168/jds.S0022-0302(05)73006-X
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
Ruminal biohydrogenation of alpha-linolenic acid is not fully understood compared with that of linoleic acid. Some hypothetical intermediates, that is, conjugated isomers of alpha-linolenic acid (cis-9, trans-11, cis-15 and cis-9, trans-13, cis-15 18: 3) have never been reported to occur in ruminant fat. Therefore, milk fat was analyzed using a combination of techniques to characterize alpha-linolenic acid biohydrogenation intermediates. Tandem off-line argentation thin-layer chromatography and high-resolution gas-liquid chromatography using a 120-m highly polar, open tubular capillary column coated with 70% cyanoalkyl polysiloxane equivalent material was used for quantification. Structural characterization of fatty acids was achieved by gas-chromatography mass-spectrometry after synthesis of specific azo-derivatives. This study confirmed that minute amounts of alpha-linolenic acid biohydrogenation intermediates are present in milk fat. Routes involved in biohydrogenation of linoleic and alpha-linolenic acids in the rumen and subsequent endogenous metabolism of related biohydrogenation products are discussed.
引用
收藏
页码:3231 / 3239
页数:9
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