Improved Proteome and Phosphoproteome Analysis on a Cation Exchanger by a Combined Acid and Salt Gradient

被引：65

作者：

Adachi, Jun ^{[1
]}

Hashiguchi, Kazunari ^{[1
,3
]}

Nagano, Maiko ^{[1
]}

Sato, Misako ^{[1
,4
]}

Sato, Ayako ^{[1
]}

Fukamizu, Kazuna ^{[1
]}

Ishihama, Yasushi ^{[2
]}

Tomonaga, Takeshi ^{[1
]}

机构：

[1] Natl Inst Biomed Innovat Hlth & Nutr, Lab Proteome Res, Osaka 5670085, Japan

[2] Kyoto Univ, Grad Sch Pharmaceut Sci, Kyoto 6068501, Japan

[3] Fukuoka Dent Coll, Div Biomed Sci, Fukuoka 8140193, Japan

[4] Osaka City Univ, Grad Sch Med, Osaka 5458586, Japan

来源：

ANALYTICAL CHEMISTRY | 2016年 / 88卷 / 16期

关键词：

MASS-SPECTROMETRY; ENRICHMENT; PHOSPHORYLATION; FRACTIONATION; DYNAMICS; DRAFT;

D O I：

10.1021/acs.analchem.6b01232

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学];

摘要：

Currently used elution methods for strong cation exchange (SCX) chromatography are based on two principles: salt arid pH gradient. In this paper, we report the first observation of peptide elution by acid gradient. The degree of peptide separation using C18-SCX StageTip was greatly improved hy our acid and salt-based elution method compared with a salt-based elution method. This development enabled us to identify over 22 000 phosphopeptides from 2 mg of protein without labor-intensive sample preparation. Our method is simple, robust, scalable, and low-cost and can be easily implemented without any special equipment or techniques.

引用

页码：7899 / 7903

页数：5

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