Genetic analysis of lysosomal trafficking in Caenorhabditis elegans

被引：198

作者：

Hermann, GJ ^{[1
]}

Schroeder, LK

Hieb, CA

Kershner, AM

Rabbitts, BM

Fonarev, P

Grant, BD

Priess, JR

机构：

[1] Lewis & Clark Coll, Dept Biol, Portland, OR 97219 USA

[2] Rutgers State Univ, Dept Mol Biol & Biochem, Piscataway, NJ 08854 USA

[3] Fred Hutchinson Canc Res Ctr, Div Basic Sci, Seattle, WA 98109 USA

[4] Univ Washington, Dept Zool, Seattle, WA 98109 USA

[5] Howard Hughes Med Inst, Seattle, WA 98109 USA

来源：

MOLECULAR BIOLOGY OF THE CELL | 2005年 / 16卷 / 07期

关键词：

D O I：

10.1091/mbc.E05-01-0060

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The intestinal cells of Caenorhabditis elegans embryos contain prominent, birefringent gut granules that we show are lysosome-related organelles. Gut granules are labeled by lysosomal markers, and their formation is disrupted in embryos depleted of AP-3 subunits, VPS-16, and VPS-41. We define a class of gut granule loss (glo) mutants that are defective in gut granule biogenesis. We show that the glo-1 gene encodes a predicted Rab GTPase that localizes to lysosome-related gut granules in the intestine and that glo-4 encodes a possible GLO-1 guanine nucleotide exchange factor. These and other glo genes are homologous to genes implicated in the biogenesis of specialized lysosome-related organelles such as melanosomes in mammals and pigment granules in Drosophila. The glo mutants thus provide a simple model system for the analysis of lysosome-related organelle biogenesis in animal cells.

引用

页码：3273 / 3288

页数：16

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