Pentapeptide scanning mutagenesis: encouraging old proteins to execute unusual tricks

被引：34

作者：

Hayes, F

Hallet, B

机构：

[1] Univ Manchester, Inst Sci & Technol, Dept Biomol Sci, Manchester M60 1QD, Lancs, England

[2] Catholic Univ Louvain, Unite Genet, Dept Biol, B-1348 Louvain, Belgium

来源：

TRENDS IN MICROBIOLOGY | 2000年 / 8卷 / 12期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1016/S0966-842X(00)01857-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Pentapeptide scanning mutagenesis is a facile transposon-based procedure for the random insertion of a variable five amino acid cassette into a target protein. The analysis of a library of proteins harbouring pentapeptide insertions can provide invaluable information on the essential and inessential regions of a target protein, as well as revealing surprising aspects of target protein function and activity.

引用

收藏

页码：571 / 577

页数：9

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