Characterization and anti-tumor activity of a polysaccharide from Hedysarum polybotrys Hand.-Mazz

被引：136

作者：

Li, Shi-gang ^{[2
]}

Wang, De-gui ^{[1
]}

Tian, Wei ^{[3
]}

Wang, Xue-xi ^{[1
]}

Zhao, Jian-xiong ^{[1
]}

Liu, Zhong ^{[1
]}

Chen, Ru ^{[1
]}

机构：

[1] Lanzhou Univ, Inst Basic Med, Lanzhou 730000, Peoples R China

[2] China Three Gorges Univ, Inst Basic Med, Yichang 443002, Peoples R China

[3] Lanzhou Univ, State Lab Appl Organ Chem, Lanzhou 730000, Peoples R China

来源：

CARBOHYDRATE POLYMERS | 2008年 / 73卷 / 02期

关键词：

Hedysarum polybotrys Hand.-Mazz; D-glucan; structure; anti-tumor;

D O I：

10.1016/j.carbpol.2007.12.001

中图分类号：

O69 [应用化学];

学科分类号：

081704 [应用化学];

摘要：

A water-soluble polysaccharide named as HPS-1 was isolated from the roots of Hedysarum polybotrys Hand.-Mazz by hot water extraction, anion-exchange and gel-permeation chromatography and tested for its anti-tumor activity. Its structural characteristics were investigated by FTIR, HPLC, NMR spectroscopy, GLC-MS, methylation analysis, periodate oxidation and Smith degradation. Based on the data obtained, HPS-1 was found to be an alpha-(1 -> 4)-D-glucan, with a single alpha-D-glucose at the C-6 position every nine residue, on average, along the main chain. The glucan has a weight-average molecular weight of about 9.4 x 10(4) Da. 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay revealed that HPS-1 significantly inhibited the proliferation of human hepatocellular carcinoma HEP-G2 cells and human gastric cancer MGC-803 cells in vitro, indicating HPS-1 could have a possible cancer therapeutic potential. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：344 / 350

页数：7

共 36 条

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