Molecular cloning and characterization of rKAB1, which interacts with KARP-1, localizes in the nucleus and protects cells against oxidative death

被引：4

作者：

Do, EJ ^{[1
]}

Taira, E ^{[1
]}

Irie, Y ^{[1
]}

Gan, YH ^{[1
]}

Tanaka, H ^{[1
]}

Kuo, CH ^{[1
]}

Miki, N ^{[1
]}

机构：

[1] Osaka Univ, Sch Med, Dept Pharmacol, Osaka 5650871, Japan

来源：

MOLECULAR AND CELLULAR BIOCHEMISTRY | 2003年 / 248卷 / 1-2期

关键词：

rKAB1; KARP-1; DNA-PK; oxidative death;

D O I：

10.1023/A:1024157515342

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The Ku autoantigen/KARP-1 (Ku86 autoantigen related protein-1) plays an important role in the double-strand break repair of mammalian DNA as a DNA-binding component of DNA-dependent protein kinase (DNA-PK) complex. KARP-1 is differently transcribed from the human Ku86 autoantigen gene locus and it is implicated in the control of DNA-dependent protein kinase activity. We cloned rKAB1, a rat homolog of KAB1 (KARP-1 binding protein 1 of human) from a rat hippocampal cDNA library. rKAB1 mRNA was specifically expressed in the brain and the thymus. EGFP-tagged rKAB1 protein localized in cell nucleus and in the condensed chromosome during the mitotic cell division. We found that rKAB1 works as a protective protein against cell damage by oxidative stress.

引用

页码：77 / 83

页数：7

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