Messenger RNA conformations in the ribosomal E site revealed by X-ray crystallography

被引：49

作者：

Jenner, Lasse ^{[1
]}

Rees, Bernard ^{[1
]}

Yusupov, Marat ^{[1
]}

Yusupova, Gulnara ^{[1
]}

机构：

[1] Inst Genet & Biol Mol & Cellulaire, F-67404 Illkirch Graffenstaden, France

来源：

EMBO REPORTS | 2007年 / 8卷 / 09期

关键词：

ribosome; tRNA; mRNA; structure; E-codon;

D O I：

10.1038/sj.embor.7401044

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A comparison of messenger RNA in X-ray crystal structures of 70S ribosomal complexes in the initiation, post-initiation and elongation states of translation shows distinct conformational differences in the exit ( E) codon. Here, we present structural evidence indicating that, after the initiation event, the E codon nucleotides relax and form a classical A-helical conformation. This conformation is similar to that of the P and A codons, and is favourable for establishing Watson-Crick interactions with the anticodon of E-site transfer RNA.

引用

页码：846 / 850

页数：5

共 29 条

[1] Visualization of tRNA movements on the Escherichia coli 70S ribosome during the elongation cycle [J].

Agrawal, RK ;

Spahn, CMT ;

Penczek, P ;

Grassucci, RA ;

Nierhaus, KH ;

Frank, J .

JOURNAL OF CELL BIOLOGY, 2000, 150 (03) :447-459

[2] Direct visualization of A-, P-, and E-site transfer RNAs in the Escherichia coli ribosome [J].

Agrawal, RK ;

Penczek, P ;

Grassucci, RA ;

Li, YH ;

Leith, A ;

Nierhaus, KH ;

Frank, J .

SCIENCE, 1996, 271 (5251) :1000-1002

[3] Structural basis for mRNA and tRNA positioning on the ribosome [J].

Berk, Veysel ;

Zhang, Wen ;

Pai, Raj D. ;

Cate, Jamie H. Doudna .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2006, 103 (43) :15830-15834

[4] Interactions between 23S rRNA and tRNA in the ribosomal E site [J].

Bocchetta, M ;

Xiong, L ;

Shah, S ;

Mankin, AS .

RNA, 2001, 7 (01) :54-63

[5] Crystallography & NMR system:: A new software suite for macromolecular structure determination [J].

Brunger, AT ;

Adams, PD ;

Clore, GM ;

DeLano, WL ;

Gros, P ;

Grosse-Kunstleve, RW ;

Jiang, JS ;

Kuszewski, J ;

Nilges, M ;

Pannu, NS ;

Read, RJ ;

Rice, LM ;

Simonson, T ;

Warren, GL .

ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY, 1998, 54 :905-921

[6]

DeLano W. L., 2002, PYMOL

[7] SIGNIFICANCE OF THE 3RD TRANSFER-RNA BINDING-SITE, THE E SITE, ON ESCHERICHIA-COLI RIBOSOMES FOR THE ACCURACY OF TRANSLATION - AN OCCUPIED E SITE PREVENTS THE BINDING OF NON-COGNATE AMINOACYL-TRANSFER RNA TO THE A SITE [J].

GEIGENMULLER, U ;

NIERHAUS, KH .

EMBO JOURNAL, 1990, 9 (13) :4527-4533

[8]

GOGIYA Z B, 1986, Molekulyarnaya Biologiya (Moscow), V20, P519

[9] Translational operator of mRNA on the ribosome: How repressor proteins exclude ribosome binding [J].

Jenner, L ;

Romby, P ;

Rees, B ;

Schulze-Briese, C ;

Springer, M ;

Ehresmann, C ;

Ehresmann, B ;

Moras, D ;

Yusupova, G ;

Yusupov, M .

SCIENCE, 2005, 308 (5718) :120-123

[10] Electron-density map interpretation [J].

Jones, TA ;

Kjeldgaard, M .

MACROMOLECULAR CRYSTALLOGRAPHY, PT B, 1997, 277 :173-208

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