Antibacterial Performance of Polydopamine-Modified Polymer Surfaces Containing Passive and Active Components

被引:336
作者
Sileika, Tadas S. [1 ]
Kim, Hyung-Do [1 ]
Maniak, Piotr [1 ]
Messersmith, Phillip B. [1 ,2 ,3 ,4 ,5 ,6 ]
机构
[1] Northwestern Univ, Dept Biomed Engn, Evanston, IL 60208 USA
[2] Northwestern Univ, Dept Mat Sci & Engn, Evanston, IL 60208 USA
[3] Northwestern Univ, Chem & Biol Engn Dept, Evanston, IL 60208 USA
[4] Northwestern Univ, Chem Life Proc Inst, Evanston, IL 60208 USA
[5] Northwestern Univ, Inst Bionanotechnol Med, Evanston, IL 60208 USA
[6] Northwestern Univ, Robert H Lurie Comprehens Canc Ctr, Evanston, IL 60208 USA
关键词
antifouling; antimicrobial; antibacterial; infection; silver; nanoparticle; coating; dopamine; polymer; SILVER NANOPARTICLES; BACTERIAL ADHESION; CANDIDA-ALBICANS; BIOFILMS; INFECTIONS; BRUSHES; IMMOBILIZATION; GENERATION; CATHETERS;
D O I
10.1021/am200978h
中图分类号
TB3 [工程材料学];
学科分类号
0805 ; 080502 ;
摘要
A growing number of device-related nosocomial infections, elevated hospitalization costs, and patient morbidity necessitate the development of novel antibacterial strategies for clinical devices. We have previously demonstrated a simple, aqueous polydopamine dip-coating method to functionalize surfaces for a wide variety of uses. Here, we extend this strategy with the goal of imparting antifouling and antimicrobial properties to substrates, exploiting the ability of polydopamine to immobilize polymers and induce metal nanoparticle formation. Polydopamine was deposited as a thin adherent film of 4 nm thickness from alkaline aqueous solution onto polycarbonate substrates, followed by grafting of antifouling polymer polyethylene glycol and in situ deposition of silver nanoparticles onto the polydopamine coated polycarbonate substrates. Elemental and morphological surface analyses confirmed successful grafting of polyethylene glycol brushes onto polydopamine-coated substrates, as well as spontaneous silver nanoparticle formation for polydopamine-coated substrates incubated in silver-nitrate solutions. Sustained silver release was observed over at least 7 days from silver-coated substrates, and the release kinetics could be modulated via additional polydopamine overlayers. In vitro functional assays employing gram negative and positive strains demonstrated dual fouling resistance and antibacterial properties of the coatings due to the fouling resistance of grafted polyethylene glycol and antibacterial effect of silver, respectively. Polycarbonate substrates coated only with silver using a method similar to existing commercial coatings provided an antibacterial effect but failed to inhibit bacterial attachment. Taking into account the previously demonstrated substrate versatility of polydopamine coatings, our findings suggest that this strategy could be implemented on a variety of substrate materials to simultaneously improve antifouling and antimicrobial performance.
引用
收藏
页码:4602 / 4610
页数:9
相关论文
共 43 条
[1]  
ARTHUR LK, 2001, BACT GROWTH FORM, P396
[2]   Antifouling Coatings: Recent Developments in the Design of Surfaces That Prevent Fouling by Proteins, Bacteria, and Marine Organisms [J].
Banerjee, Indrani ;
Pangule, Ravindra C. ;
Kane, Ravi S. .
ADVANCED MATERIALS, 2011, 23 (06) :690-718
[3]   Bacterial biofilms: A common cause of persistent infections [J].
Costerton, JW ;
Stewart, PS ;
Greenberg, EP .
SCIENCE, 1999, 284 (5418) :1318-1322
[4]   Mussel adhesive protein mimetic polymers for the preparation of nonfouling surfaces [J].
Dalsin, JL ;
Hu, BH ;
Lee, BP ;
Messersmith, PB .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2003, 125 (14) :4253-4258
[5]   Eliminating biofilm from ureteral stents: the holy grail [J].
Denstedt, John D. ;
Cadieux, Peter A. .
CURRENT OPINION IN UROLOGY, 2009, 19 (02) :205-210
[6]   Biofilms and device-associated infections [J].
Donlan, RM .
EMERGING INFECTIOUS DISEASES, 2001, 7 (02) :277-281
[7]   Biofilms: Survival mechanisms of clinically relevant microorganisms [J].
Donlan, RM ;
Costerton, JW .
CLINICAL MICROBIOLOGY REVIEWS, 2002, 15 (02) :167-+
[8]   Protein-induced changes in poly(ethylene glycol) brushes: Molecular weight and temperature dependence [J].
Efremova, NV ;
Sheth, SR ;
Leckband, DE .
LANGMUIR, 2001, 17 (24) :7628-7636
[9]   Cell fouling resistance of polymer brushes grafted from Ti substrates by surface-initiated polymerization: Effect of ethylene glycol side chain length [J].
Fan, Xiaowu ;
Lin, Lijun ;
Messersmith, Phillip B. .
BIOMACROMOLECULES, 2006, 7 (08) :2443-2448
[10]   The inhibition of the adhesion of clinically isolated bacterial strains on multi-component cross-linked poly(ethylene glycol)-based polymer coatings [J].
Fernandez, Isabel C. Saldarriaga ;
van der Mei, Henny C. ;
Lochhead, Michael J. ;
Grainger, David W. ;
Busscher, Henk J. .
BIOMATERIALS, 2007, 28 (28) :4105-4112