Characterization of a cDNA encoding a novel avian hypothalamic neuropeptide exerting an inhibitory effect on gonadotropin release

被引:166
作者
Satake, H
Hisada, M
Kawada, T
Minakata, H
Ukena, K
Tsutsui, K [1 ]
机构
[1] Hiroshima Univ, Fac Integrated Arts & Sci, Lab Brain Sci, Higashihiroshima 7398521, Japan
[2] Suntory Inst Bioorgan Res, Shimamoto, Osaka 6188503, Japan
关键词
gonadotropin inhibitory hormone; mass spectrometry; quail;
D O I
10.1042/0264-6021:3540379
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We previously isolated a novel dodecapeptide containing a C-terminal -Arg-Phe-NH2 sequence, SIKPSAYLPLRF-NH2 (RFamide peptide), from the quail brain. This quail RFamide peptide was shown to decrease gonadotropin release from the cultured anterior pituitary and to be located at least in the quail hypothalamo-hypophysial system. We therefore designated this RFamide peptide gonadotropin inhibitory hormone (GnIH). In the present study we characterized the GnIH cDNA from the quail brain by a combination of 3' and 5' rapid amplification of cDNA. ends ('RACE'). The deduced GnIH precursor consisted of 173 amino acid residues, encoding one GnIH and two putative gene-related peptide (GnIH-RP-1 and GnIH-RP-2) sequences that included -LPXRF (X = L or Q) at their C-termini. All these peptide sequences were flanked by a glycine C-terminal amidation signal and a single basic amino acid on each end as an endoproteolytic site. Southern blotting analysis of reverse-transcriptase-mediated PCR products demonstrated a specific expression of the gene encoding GnIH in the diencephalon including the hypothalamus. Furthermore, mass spectrometric analyses detected the mass numbers for matured GnIH and GnIH-RP-2, revealing that both peptides are produced from the precursor in the diencephalon as an endogenous ligand. Taken together, these results lead to the conclusion that GnIH is a hypothalamic factor responsible for the negative regulation of gonadotropin secretion. Furthermore, the presence of a novel RFamide peptide family containing a C-terminal -LPXRF-NH2 sequence has been revealed.
引用
收藏
页码:379 / 385
页数:7
相关论文
共 35 条
[11]   Expression of gonadotropin-releasing hormone and gonadotropin-releasing hormone receptor mRNAs in various non-reproductive human tissues [J].
Kakar, SS ;
Jennes, L .
CANCER LETTERS, 1995, 98 (01) :57-62
[12]  
KING JA, 1982, J BIOL CHEM, V257, P722
[13]  
Klumperman J, 1996, J NEUROSCI, V16, P7930
[14]   THE NUCLEOTIDE-SEQUENCE OF THE CHICK CYTOPLASMIC BETA-ACTIN GENE [J].
KOST, TA ;
THEODORAKIS, N ;
HUGHES, SH .
NUCLEIC ACIDS RESEARCH, 1983, 11 (23) :8287-8301
[15]   AN ANALYSIS OF 5'-NONCODING SEQUENCES FROM 699 VERTEBRATE MESSENGER-RNAS [J].
KOZAK, M .
NUCLEIC ACIDS RESEARCH, 1987, 15 (20) :8125-8148
[16]   STRUCTURE OF PORCINE LH- AND FSH-RELEASING HORMONE .1. PROPOSED AMINO ACID SEQUENCE [J].
MATSUO, H ;
BABA, Y ;
NAIR, RMG ;
ARIMURA, A ;
SCHALLY, AV .
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1971, 43 (06) :1334-+
[17]   IDENTIFICATION OF THE 2ND GONADOTROPIN-RELEASING HORMONE IN CHICKEN HYPOTHALAMUS - EVIDENCE THAT GONADOTROPIN-SECRETION IS PROBABLY CONTROLLED BY 2 DISTINCT GONADOTROPIN-RELEASING HORMONES IN AVIAN SPECIES [J].
MIYAMOTO, K ;
HASEGAWA, Y ;
NOMURA, M ;
IGARASHI, M ;
KANGAWA, K ;
MATSUO, H .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (12) :3874-3878
[18]   Furin: a mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins [J].
Nakayama, K .
BIOCHEMICAL JOURNAL, 1997, 327 :625-635
[19]   Neuroregulation of ProTRH biosynthesis and processing [J].
Nillni, EA .
ENDOCRINE, 1999, 10 (03) :185-199
[20]   Procorticotrophin-releasing hormone: endoproteolytic processing and differential release of its derived peptides within AtT20 cells [J].
Perone, MJ ;
Murray, CA ;
Brown, OA ;
Gibson, S ;
White, A ;
Linton, EA ;
Perkins, AV ;
Lowenstein, PR ;
Castro, MG .
MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1998, 142 (1-2) :191-202