A water-soluble tetrazolium salt useful for colorimetric cell viability assay

被引：573

作者：

Tominaga, H

Ishiyama, M

Ohseto, F

Sasamoto, K

Hamamoto, T

Suzuki, K

Watanabe, M

机构：

[1] Dojindo Labs, Kumamoto 8612202, Japan

[2] Nagasaki Univ, Sch Med, Dept Hosp Pharm, Nagasaki 8528501, Japan

[3] Nagasaki Univ, Sch Pharmaceut Sci, Dept Hlth Sci, Nagasaki 8528131, Japan

来源：

ANALYTICAL COMMUNICATIONS | 1999年 / 36卷 / 02期

关键词：

D O I：

10.1039/a809656b

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The application of a tetrazolium salt, 2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H tetrazolium, monosodium salt (WST-8), to cell viability assays and in vitro drug sensitivity tests is described. With a higher sensitivity as a chromogenic indicator for cell viability compared with conventional tetrazolium salts, WST-8 produced results of cell viability and IC50 values that were in good agreement, respectively, with the MTT method and [H-3]thymidine uptake method.

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页码：47 / 50

页数：4

相关论文

共 20 条

[1] TOXICITY DETERMINED INVITRO BY MORPHOLOGICAL ALTERATIONS AND NEUTRAL RED ABSORPTION [J].

BORENFREUND, E ;

PUERNER, JA .

TOXICOLOGY LETTERS, 1985, 24 (2-3) :119-124

[2] USE OF AN AQUEOUS SOLUBLE TETRAZOLIUM FORMAZAN ASSAY FOR CELL-GROWTH ASSAYS IN CULTURE [J].

CORY, AH ;

OWEN, TC ;

BARLTROP, JA ;

CORY, JG .

CANCER COMMUNICATIONS, 1991, 3 (07) :207-212

[3] Enhanced protection against peroxidation-induced mortality of aortic endothelial cells by ascorbic acid 2-O-phosphate abundantly accumulated in the cell as the dephosphorylated form [J].

Fujiwara, M ;

Nagao, N ;

Monden, K ;

Misumi, M ;

Kageyama, K ;

Yamamoto, K ;

Miwa, N .

FREE RADICAL RESEARCH, 1997, 27 (01) :97-&

[4] TGF-beta-mediated hepatocellular apoptosis by rat and human hepatoma cells and primary rat hepatocytes [J].

Gressner, AM ;

Lahme, B ;

Mannherz, HG ;

Polzar, B .

JOURNAL OF HEPATOLOGY, 1997, 26 (05) :1079-1092

[5] Attenuation of TGF-beta-induced apoptosis in primary cultures of hepatocytes by calpain inhibitors [J].

Gressner, AM ;

Lahme, B ;

Roth, S .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 231 (02) :457-462

[6] A biological method for the quantitative measurement of tetrodotoxin (TTX): Tissue culture bioassay in combination with a water-soluble tetrazolium salt [J].

Hamasaki, K ;

Kogure, K ;

Ohwada, K .

TOXICON, 1996, 34 (04) :490-495

[7]

Heiss P, 1997, ANTICANCER RES, V17, P3177

[8]

ISHIYAMA M, 1993, CHEM PHARM BULL, V41, P1118, DOI 10.1248/cpb.41.1118

[9]

Ishiyama M, 1996, BIOL PHARM BULL, V19, P1518

[10] A highly water-soluble disulfonated tetrazolium salt as a chromogenic indicator for NADH as well as cell viability [J].

Ishiyama, M ;

Miyazono, Y ;

Sasamoto, K ;

Ohkura, Y ;

Ueno, K .

TALANTA, 1997, 44 (07) :1299-1305