Primary differentiation in the human blastocyst: Comparative molecular portraits of inner cell mass and trophectoderm cells

被引:175
作者
Adjaye, J
Huntriss, J
Herwig, R
BenKahla, A
Brink, TC
Wierling, C
Hultschig, C
Groth, D
Yaspo, ML
Picton, HM
Gosden, RG
Lehrach, H
机构
[1] Max Planck Inst Mol Genet, Dept Vertebrate Genom, D-14195 Berlin, Germany
[2] Univ Leeds, Reprod & Early Dev Res Grp, Dept Obstet & Gynaecol, Leeds, W Yorkshire, England
[3] Cornell Univ, Weill Med Coll, Ctr Reprod Med & Infertil, New York, NY USA
关键词
preimplantation development; blastocyst; inner cell mass; trophectoderin; pluripotency; embryonic stem cells; trophoblastic stem cells; OCT4; CDX2; differentiation; microarrays;
D O I
10.1634/stemcells.2005-0113
中图分类号
Q813 [细胞工程];
学科分类号
摘要
The primary differentiation event during mammalian development occurs at the blastocyst stage and leads to the delineation of the inner cell mass (ICM) and the trophectoderm (TE). We provide the first global mRNA expression data from immunosurgically dissected ICM cells, TE cells, and intact human blastocysts. Using a cDNA microarray composed of 15,529 cDNAs from known and novel genes, we identify marker transcripts specific to the 1CM (e.g., OCT4/POU5F1, NANOG, HMGB1, and DPPA5) and TE (e.g., CDX2, ATP1B3, SFN, and IPL), in addition to novel ICM- and TE-specific expressed sequence tags. The expression patterns suggest that the emergence of pluripotent ICM and TE cell lineages from the morula is controlled by metabolic and signaling pathways, which include inter alia, WNT, mitogen-activated protein kinase, transforming growth factor-beta, NOTCH, integrin-mediated cell adhesion, phosphatidylinositol 3-kinase, and apoptosis. These data enhance our understanding of the first step in human cellular differentiation and, hence, the derivation of both embryonic stem cells and trophoblastic stem cells from these lineages. STEM CELLS 2005;23:1514-1525.
引用
收藏
页码:1514 / 1525
页数:12
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