Analysis of site-specific phosphorylation of the retinoblastoma protein during cell cycle progression

被引:30
作者
Boylan, JF [1 ]
Sharp, DM [1 ]
Leffet, L [1 ]
Bowers, A [1 ]
Pan, WJ [1 ]
机构
[1] Dupont Merck Pharmaceut Co, Genet & Canc Grp, Wilmington, DE 19880 USA
关键词
cell cycle; retinoblastoma protein; antibody;
D O I
10.1006/excr.1999.4389
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Differential phosphorylation of the retinoblastoma protein plays a pivotal role in cell cycle regulation. The retinoblastoma protein is specifically phosphorylated during the cell cycle by cyclin-dependent kinase complexes which intersect with many cellular signaling networks. Since the loss of the retinoblastoma signaling pathways occurs in a wide variety of human tumors, understanding the significance of site-specific phosphorylation can clarify the role of selected cyclin-dependent kinase complexes during cell cycle progression. Here we describe the phosphospecificity and cellular characterization of a panel of polyclonal antibodies that recognize unique phosphorylation sites within the retinoblastoma protein. These reagents were used to validate authentic cellular retinoblastoma phosphorylation sites at amino acids 780, 795, and 807/811. correlating with the G1-S transition. (C) 1999 Academic Press.
引用
收藏
页码:110 / 114
页数:5
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