Development of an ELISA for sulfachlorpyridazine and investigation of matrix effects from different sample extraction procedures

被引:31
作者
Spinks, CA
Schut, CG
Wyatt, GM
Morgan, MRA
机构
[1] Inst Food Res, Diet Hlth & Consumer Sci Div, Norwich NR4 7UA, Norfolk, England
[2] Univ Leeds, Procter Dept Food Sci, Leeds LS2 9JT, W Yorkshire, England
来源
FOOD ADDITIVES AND CONTAMINANTS | 2001年 / 18卷 / 01期
关键词
ELISA; sulfachlorpyridazine; matrix effects; extraction;
D O I
10.1080/02652030010002630
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
The development of an enzyme-linked immunosorbent assay (ELISA) for the detection of residues of sulfachlorpyridazine (SCP) is described for the first time. The assay is highly specific for SCP, is simple to perform and has a lower detection limit of 0.65 ng/ml in assay buffer. The potential application of the assay to detect residues of SCP at the 0.1 mg/kg level in eggs, milk, beef, lamb, pork, chicken, turkey, porcine kidney, porcine liver and pig feedstuffs is discussed with regard to the effects of sample extracts on the standard curves. The antibody exhibits a rare stability in assay buffers containing up to 30% methanol. It is concluded that the ELISA for SCP has the appropriate characteristics for development into a robust method for the detection of this sulphonamide in agri-food materials.
引用
收藏
页码:11 / 18
页数:8
相关论文
共 33 条
[1]   DETERMINATION OF SULFONAMIDES IN PORK MEAT EXTRACTS BY CAPILLARY ZONE ELECTROPHORESIS [J].
ACKERMANS, MT ;
BECKERS, JL ;
EVERAERTS, FM ;
HOOGLAND, H ;
TOMASSEN, MJH .
JOURNAL OF CHROMATOGRAPHY, 1992, 596 (01) :101-109
[2]  
BARKER SA, 1994, J AOAC INT, V77, P848
[3]  
BLANCHFLOWER WJ, 1988, J ASSOC OFF ANA CHEM, V71, P302
[4]   Gas chromatographic mass spectrometric determination of sulfamethazine in animal tissues using a methyl/trimethylsilyl derivative [J].
Cannavan, A ;
Hewitt, SA ;
Blanchflower, WJ ;
Kennedy, DG .
ANALYST, 1996, 121 (10) :1457-1461
[5]   DEVELOPMENT OF MULTI-RESIDUE METHODOLOGY FOR THE HPLC DETERMINATION OF VETERINARY DRUGS IN ANIMAL-TISSUES [J].
COOPER, AD ;
CREASER, CS ;
FARRINGTON, WHH ;
TARBIN, JA ;
SHEARER, G .
FOOD ADDITIVES AND CONTAMINANTS, 1995, 12 (02) :167-176
[6]  
DIXONHOLLAND DE, 1989, J ASSOC OFF ANA CHEM, V72, P447
[7]  
DIXONHOLLAND DE, 1992, ANALYSIS OF ANTIBIOTIC / DRUG RESIDUES IN FOOD PRODUCTS OF ANIMAL ORIGIN, P57
[8]  
ENDOH YS, 1994, J FOOD HYG SOC JPN, V35, P292, DOI 10.3358/shokueishi.35.292
[9]  
FINGLAS PM, 1994, FOOD CHEM, V49, P192
[10]  
FLEEKER JR, 1985, J ASSOC OFF ANA CHEM, V68, P172