Pharmacological characterization of micropatterned cardiac myocytes

被引：27

作者：

Kaji, H ^{[1
]}

Takii, Y ^{[1
]}

Nishizawa, M ^{[1
]}

Matsue, T ^{[1
]}

机构：

[1] Tohoku Univ, Grad Sch Engn, Dept Biomol Engn, Sendai, Miyagi 9808579, Japan

来源：

BIOMATERIALS | 2003年 / 24卷 / 23期

关键词：

micropatterning; cardiomyocyte; cell adhesion; polydimethylsiloxane; fibronectin;

D O I：

10.1016/S0142-9612(03)00275-8

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

The cardiac myocytes patterned on a single cell level were prepared by microcontact printing, and the response to chemical stimuli was studied using confocal fluorescent Ca2+ imaging. The patterned iriyocytes were found to conjugate by forming gap junction. It was confirmed for the patterned myocytes that gap junction communication was reversibly inhibited by 1-octanol, but activated by caffeine. Localized stimulation with chemicals was also attempted using a microinjection system for the myocyte patterns formed by single cell alignment. This research was carried out with the objective of developing a bioassay system based on a cellular network. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：4239 / 4244

页数：6

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