Towards the conception of an amperometric sensor of L-tyrosine based on Hemin/PAMAM/MWCNT modified glassy carbon electrode

被引:80
作者
Ma, Qiang [1 ]
Ai, Shiyun [1 ]
Yin, Huanshun [1 ]
Chen, Quanpeng [2 ]
Tang, Tiantian [2 ]
机构
[1] Shandong Agr Univ, Coll Chem & Mat Sci, Tai An 271018, Shandong, Peoples R China
[2] Shandong Agr Univ, Coll Resources & Environm, Tai An 271018, Shandong, Peoples R China
基金
中国国家自然科学基金;
关键词
Amperometric sensor; Hemin; L-Tyrosine; Poly (amidoamine); Multi-walled carbon nanotube; ELECTROCHEMICAL-BEHAVIOR; HYDROGEN-PEROXIDE; PROTOPORPHYRIN-IX; DENDRITIC MACROMOLECULES; STARBURST DENDRIMERS; GOLD ELECTRODE; HEMIN; NANOTUBES; FILMS; NITRITE;
D O I
10.1016/j.electacta.2010.06.003
中图分类号
O646 [电化学、电解、磁化学];
学科分类号
081704 ;
摘要
A novel amperometric sensor was fabricated based on the immobilization of hemin onto the poly (amidoamine)/multi-walled carbon nanotube (PAMAM/MWCNT) nanocomposite film modified glassy carbon electrode (GCE). Electrochemical impedance spectroscopy (EIS), cyclic voltammetry (CV) and ultraviolet visible (UV-vis) adsorption spectroscopy were used to investigate the possible state and electrochemical activity of the immobilized hemin. In the Hemin/PAMAM/MWCNT nanocomposite film, MWCNT layer possessed excellent inherent conductivity to enhance the electron transfer rate, while the layer of PAMAM greatly enlarged the surface average concentration of hemin (Gamma) on the modified electrode. Therefore, the nanocomposite film showed enhanced electrocatalytical activity towards the oxidation of L-tyrosine. The kinetic parameters of the modified electrode were investigated. In pH 7.0 phosphate buffer solution (PBS), the sensor exhibits a wide linear range from 0.1 mu M to 28.8 mu M L-tyrosine with a detection limit of 0.01 mu M and a high sensitivity of 0.31 mu A mu M-1 cm(-2). In addition, the response time of the L-tyrosine sensor is less than 5s. The excellent performance of the sensor is largely attributed to the electro-generated high reactive oxoiron (IV) porphyrin (O = Fe-IV-P) which effectively catalyzed the oxidation of L-tyrosine. A mechanism was herein proposed for the catalytic oxidation of L-tyrosine by oxoiron (IV) porphyrin complexes. (C) 2010 Elsevier Ltd. All rights reserved.
引用
收藏
页码:6687 / 6694
页数:8
相关论文
共 58 条
[51]   Application of multi-walled carbon nanotubes functionalized with hemin for oxygen detection in neutral solution [J].
Ye, JS ;
Wen, Y ;
De Zhang, W ;
Cui, HF ;
Gan, LM ;
Xu, GQ ;
Sheu, FS .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2004, 562 (02) :241-246
[52]   Electrochemical behavior and determination of L-tyrosine at single-walled carbon nanotubes modified glassy carbon electrode [J].
Yu, Xiaozhi ;
Mai, Zhibin ;
Xiao, Yun ;
Zou, Xiaoyong .
ELECTROANALYSIS, 2008, 20 (11) :1246-1251
[53]   Acylpyrazolone Modified Glassy Carbon Electrode Effect on the Electrochemical Behavior of Tyrosine [J].
Zhang Dan ;
Li Jin-zhou .
ANALYTICAL LETTERS, 2008, 41 (15) :2832-2843
[54]   Fabrication of multi-wall carbon nanotube film on glassy carbon electrode surface and the determination of tyrosine [J].
Zhang, SH ;
Qu, WY ;
Huang, WS ;
Wu, YY .
JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, 2004, 4 (05) :553-557
[55]   An ionic liquid supported CeO2 nanoshuttles-carbon nanotubes composite as a platform for impedance DNA hybridization sensing [J].
Zhang, Wei ;
Yang, Tao ;
Zhuang, Xuming ;
Guo, Zhiyan ;
Jiao, Kui .
BIOSENSORS & BIOELECTRONICS, 2009, 24 (08) :2417-2422
[56]   Cupreous oxide nanobelts as detector for determination of L-Tyrosine [J].
Zhang, Xiaojun ;
Wang, Guangfeng ;
Wang, Qi ;
Zhao, Lijun ;
Wang, Man ;
Fang, Bin .
MATERIALS SCIENCE AND ENGINEERING B-ADVANCED FUNCTIONAL SOLID-STATE MATERIALS, 2009, 156 (1-3) :6-9
[57]  
Zhao GH, 2006, ELECTROANAL, V18, P830, DOI 10.1002/elan.200503455
[58]   Study of the adsorption kinetics of thiol-derivatized porphyrin on the surface of gold electrode [J].
Zuo, Guofang ;
Liu, Xiuhui ;
Yang, Jiandong ;
Li, Xiujuan ;
Lu, Xiaoquan .
JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 2007, 605 (02) :81-88