A novel canine lymphoma cell line: A translational and comparative model for lymphoma research

被引：44

作者：

Kisseberth, William C. ^{[1
,3
]}

Nadella, Murali Vara Prasad ^{[2
]}

Breen, Matthew ^{[4
,5
]}

Thomas, Rachael ^{[4
]}

Duke, Shannon E. ^{[4
]}

Murahari, Sridhar ^{[1
]}

Kosarek, Carrie E. ^{[1
]}

Vernau, William ^{[6
]}

Avery, Anne C. ^{[7
]}

Burkhard, Mary Jo ^{[2
]}

Rosol, Thomas J. ^{[2
,3
]}

机构：

[1] Ohio State Univ, Vet Teaching Hosp, Coll Vet Med, Dept Vet Clin Sci, Columbus, OH 43210 USA

[2] Ohio State Univ, Coll Vet Med, Dept Vet Biosci, Columbus, OH 43210 USA

[3] Ohio State Univ, Ctr Comprehens Canc, Columbus, OH 43210 USA

[4] N Carolina State Univ, Coll Vet Med, Dept Mol Biomed Sci, Raleigh, NC 27695 USA

[5] N Carolina State Univ, Ctr Comparat Med & Translat Res, Raleigh, NC 27695 USA

[6] Univ Calif Davis, Sch Vet Med, Dept Pathol, Davis, CA 95616 USA

[7] Colorado State Univ, Coll Vet Med & Biomed Sci, Dept Microbiol Immunol & Pathol, Ft Collins, CO 80523 USA

来源：

LEUKEMIA RESEARCH | 2007年 / 31卷 / 12期

基金：

美国国家卫生研究院;

关键词：

canine lymphoma; cell line; array comparative genomic hybridization; bioluminescent imaging; xenograft;

D O I：

10.1016/j.leukres.2007.04.003

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A novel canine lymphoma cell line, OSW, was established from the malignant pleural effusion of a dog with peripheral T-cell lymphoma. The immunoprofile as determined by flow cytometry was as follows: positive for CD45, CD49d, CD18, CD11a; weakly positive for CD11b, CD11c, CD11d; and negative for CD45RA, CD1a, CD1c, CD3, TCR alpha beta, TCR gamma delta, CD4, CD5, CD8a, CD8b, CD90(Thy1), CD21, MHCII, CD14(TUK4), CD34, and MPO. Immunocytochemistry of cytospin preparations was negative for cytoplasmic CD3, CD79a, and MPO, but was positive for CD20. The cell line had an oligoclonal T-cell receptor gamma (TCR gamma) gene rearrangement. Array comparative genomic hybridization (aCGH) and single locus probe (SLP) analysis showed that there were copy number increases of loci on dog chromosome 13 (CFA 13), and copy number decreases were evident for regions of CFA 11, 22, 26, 30 and 32, which include several of the more common chromosomal aberrations reported previously in canine lymphoma. The OSW cell line grows rapidly in vitro and is tumorigenic as a xenograft in SCID/NOD mice. OSW represents one of only a few reported canine lymphoma cell lines and is the most thoroughly characterized. This cell line and xenograft represent significant in vitro and in vivo models, respectively, for comparative and translational lymphoma research. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：1709 / 1720

页数：12

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