Determination of hydroperoxides in nonaqueous solvents or mixed solvents, using a biosensor with two antagonist enzymes operating in parallel

被引：18

作者：

Campanella, L ^{[1
]}

Giancola, D ^{[1
]}

Gregori, E ^{[1
]}

Tomassetti, A ^{[1
]}

机构：

[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2003年 / 95卷 / 1-3期

关键词：

organic phase enzyme electrode; hydroperoxide; analysis;

D O I：

10.1016/S0925-4005(03)00433-7

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The development and characterisation of a new organic phase enzyme electrode (OPEE) for hydroperoxides is described. The biosensor was obtained by combining an oxygen gas diffusion amperometric electrode and two immobilised enzymes (peroxidase and tyrosinase) working in parallel and competing for the same substrate (catechol). LOD was about 0.1 X 10(-3) mol l(-1). The biosensor was applied for the determination, using decane as solvent, of the "pool" of hydroperoxides released during the heating (to about 130-140 degreesC) of extravirgin olive oil, or used to determine the hydrogen peroxide content of lipophilic cosmetic products, working in a dioxane-water mixture. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：321 / 327

页数：7

共 20 条

[1]

BOTTARI E, 1978, ANAL CHIM QUANTITATI, P267

[2] Monitoring the rancidification process in olive oils using a biosensor operating in organic solvents [J].

Campanella, L ;

Favero, G ;

Pastorino, M ;

Tomassetti, M .

BIOSENSORS & BIOELECTRONICS, 1999, 14 (02) :179-186

[3] TYROSINASE BIOSENSOR RESPONSE AS A FUNCTION OF PHYSICAL-PROPERTIES OF ORGANIC-SOLVENTS [J].

CAMPANELLA, L ;

FORTUNEY, A ;

SAMMARTINO, MP ;

TOMASSETTI, M .

TALANTA, 1994, 41 (08) :1397-1404

[4] THE EFFECT OF ORGANIC-SOLVENT PROPERTIES ON THE RESPONSE OF A TYROSINASE ENZYME SENSOR [J].

CAMPANELLA, L ;

FAVERO, G ;

SAMMARTINO, MP ;

TOMASSETTI, M .

TALANTA, 1994, 41 (06) :1015-1023

[5] Hydrogen peroxide determination in pharmaceutical formulations and cosmetics using a new catalase biosensor [J].

Campanella, L ;

Roversi, R ;

Sammartino, MP ;

Tomassetti, M .

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS, 1998, 18 (1-2) :105-116

[6]

Campanella L, 1996, ANALUSIS, V24, P288

[7] Hydroperoxide determination by a catalase OPEE: application to the study of extra virgin olive oil rancidification process [J].

Campanella, L ;

Sammartino, MP ;

Tomassetti, M ;

Zannella, S .

SENSORS AND ACTUATORS B-CHEMICAL, 2001, 76 (1-3) :158-165

[8] Organic phase enzyme electrodes: applications and theoretical studies [J].

Campanella, L ;

Favero, G ;

Persi, L ;

Sammartino, MP ;

Tomassetti, M ;

Visco, G .

ANALYTICA CHIMICA ACTA, 2001, 426 (02) :235-247

[9] Two OPEEs (organic phase enzyme electrodes) used to check the percentage water content in hydrophobic foods and drugs [J].

Campanella, L ;

De Santis, G ;

Favero, G ;

Sammartino, MP ;

Tomassetti, M .

ANALYST, 2001, 126 (11) :1923-1928

[10] A new organic phase enzyme electrode for the analysis of organophosphorus pesticides and carbamates [J].

Campanella, L ;

De Luca, S ;

Sammartino, MP ;

Tomassetti, M .

ANALYTICA CHIMICA ACTA, 1999, 385 (1-3) :59-71

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