Semipreparative capillary electrochromatography

被引：34

作者：

Chen, JR ^{[1
]}

Zare, RN ^{[1
]}

Peters, EC ^{[1
]}

Svec, F ^{[1
]}

Frechét, JJ ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

ANALYTICAL CHEMISTRY | 2001年 / 73卷 / 09期

关键词：

D O I：

10.1021/ac001302m

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Capillaries with inner diameters of 550 mum have successfully been packed with 1.5-mum octadecyl silica particles using frits made of macroporous polymers by the UV photopolymerization of a solution of glycidyl methacrylate sad trimethylolpropane trimethacrylate. This type of frit is found superior to one made of low-melting point poly(styrene-co-divinylbenzene) beads. Bubble formation is not observed to occur within these capillary columns under our experimental conditions. Separations can be achieved with sample injection volumes as high as 1 muL. To demonstrate its semipreparative use, a mixture of 500 nL of taxol (20 mM) and its precursor, baccatin III (30 mM), is separated using such a column with a Tris buffer.

引用

页码：1987 / 1992

页数：6

共 37 条

[1] Sintered octadecylsilica as monolithic column packing in capillary electrochromatography and micro high-performance liquid chromatography [J].