Maintenance of an intact human immunodeficiency virus type 1 vpr gene following mother-to-infant transmission

被引:38
作者
Yedavalli, VRK
Chappey, C
Ahmad, N [1 ]
机构
[1] Univ Arizona, Hlth Sci Ctr, Dept Microbiol & Immunol, Tucson, AZ 85724 USA
[2] NIH, Natl Lib Med, Natl Ctr Biotechnol Informat, Bethesda, MD 20894 USA
关键词
D O I
10.1128/JVI.72.8.6937-6943.1998
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The vpr sequences from six human immunodeficiency virus type 1 (HIV-1)-infected mother-infant pairs following perinatal transmission were analyzed. We found that 153 of the 166 clones analyzed from uncultured peripheral blood mononuclear cell DNA samples showed a 92.17% frequency of intact vpr open reading frames. There was a low degree of heterogeneity of vpr genes within mothers, within infants, and between epidemiologically linked mother-infant pairs. The distances between vpr sequences were greater in epidemiologically unlinked individuals than in epidemiologically linked mother-infant pairs. Moreover, the infants' sequences displayed patterns similar to those seen in their mothers. The functional domains essential for Vpr activity, including virion incorporation, nuclear import, and cell cycle arrest and differentiation were highly conserved in most of the sequences. Phylogenetic analyses of 166 mother-infant pairs and 195 other available vpr sequences from HIV databases formed distinct clusters for each mother-infant pair and for other vpr sequences and grouped the six mother-infant pairs' sequences with subtype B sequences. A high degree of conservation of intact and functional vpr supports the notion that vpr plays an important role in HIV-1 infection and replication in mother-infant isolates that are involved in perinatal transmission.
引用
收藏
页码:6937 / 6943
页数:7
相关论文
共 53 条
[41]   COMPARISON OF VARIABLE REGION-3 SEQUENCES OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 FROM INFECTED CHILDREN WITH THE RNA AND DNA-SEQUENCES OF THE VIRUS POPULATIONS OF THEIR MOTHERS [J].
SCARLATTI, G ;
LEITNER, T ;
HALAPI, E ;
WAHLBERG, J ;
MARCHISIO, P ;
CLERICISCHOELLER, MA ;
WIGZELL, H ;
FENYO, EM ;
ALBERT, J ;
UHLEN, M ;
ROSSI, P .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1993, 90 (05) :1721-1725
[42]   MOTHERS OF INFANTS WITH THE ACQUIRED IMMUNODEFICIENCY SYNDROME - EVIDENCE FOR BOTH SYMPTOMATIC AND ASYMPTOMATIC CARRIERS [J].
SCOTT, GB ;
FISCHL, MA ;
KLIMAS, N ;
FLETCHER, MA ;
DICKINSON, GM ;
LEVINE, RS ;
PARKS, WP .
JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION, 1985, 253 (03) :363-366
[43]   Gene acquisition in HIV and SIV [J].
Sharp, PM ;
Bailes, E ;
Stevenson, M ;
Emerman, M ;
Hahn, BH .
NATURE, 1996, 383 (6601) :586-587
[44]  
SHULER GD, 1996, METHOD ENZYMOL, V266, P141
[45]   Conservation and host specificity of Vpr-mediated cell cycle arrest suggest a fundamental role in primate lentivirus evolution and biology [J].
Stivahtis, GL ;
Soares, MA ;
Vodicka, MA ;
Hahn, BH ;
Emerman, M .
JOURNAL OF VIROLOGY, 1997, 71 (06) :4331-4338
[46]  
TOVO PA, 1988, LANCET, V2, P1043
[47]   Gene defects clustered at the C-terminus of the vpr gene of HIV-1 in long-term nonprogressing mother and child pair: In vivo evolution of vpr quasispecies in blood and plasma [J].
Wang, B ;
Ge, YC ;
Palasanthiran, P ;
Xiang, SH ;
Ziegler, J ;
Dwyer, DE ;
Randle, C ;
Dowton, D ;
Cunningham, A ;
Saksena, NK .
VIROLOGY, 1996, 223 (01) :224-232
[48]   HIV-1 GENOMIC RNA DIVERSIFICATION FOLLOWING SEXUAL AND PARENTERAL VIRUS TRANSMISSION [J].
WOLFS, TFW ;
ZWART, G ;
BAKKER, M ;
GOUDSMIT, J .
VIROLOGY, 1992, 189 (01) :103-110
[49]   SELECTIVE TRANSMISSION OF HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 VARIANTS FROM MOTHERS TO INFANTS [J].
WOLINSKY, SM ;
WIKE, CM ;
KORBER, BTM ;
HUTTO, C ;
PARKS, WP ;
ROSENBLUM, LL ;
KUNSTMAN, KJ ;
FURTADO, MR ;
MUNOZ, JL .
SCIENCE, 1992, 255 (5048) :1134-1137
[50]   Conservation of an intact vif gene of human immunodeficiency virus type 1 during maternal-fetal transmission [J].
Yedavalli, VRK ;
Chappey, C ;
Matala, E ;
Ahmad, N .
JOURNAL OF VIROLOGY, 1998, 72 (02) :1092-1102