Production of recombinant HIV-1/HBV virus-like particles in Nicotiana tabacum and Arabidopsis thaliana plants for a bivalent plant-based vaccine

被引：46

作者：

Greco, Raffaella ^{[2
]}

Michel, Marie ^{[1
]}

Guetard, Denise ^{[1
]}

Cervantes-Gonzalez, Minerva ^{[1
]}

Pelucchi, Nilla ^{[2
]}

Wain-Hobson, Simon ^{[1
]}

Sala, Francesco ^{[2
]}

Sala, Monica ^{[1
]}

机构：

[1] Inst Pasteur, Dept Virol, F-75015 Paris, France

[2] Univ Milan, Dept Biol, I-20133 Milan, Italy

来源：

VACCINE | 2007年 / 25卷 / 49期

关键词：

HIV-1; HBV; transgenic plants; VLPs; bivalent vaccine;

D O I：

10.1016/j.vaccine.2007.09.061

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human immunodeficiency virus (HIV-1) and hepatitis B virus (HBV) spread via similar transmission pathways, and infection by HBV occurs in up to 32% of HIV-1 cases. Here, we describe the successful expression of novel recombinant HIV-1/HBV virus-like particles (VLPs) in Nicotiana tabacum and Arabidopsis thaliana. The production levels and quality of the recombinant VLPs were comparable in the two plants, showing that parameters intrinsic to the recombinant proteins determined their assembly into VLPs. These heterologous VLPs can be used in a bivalent anti-HIV-1/-HBV vaccine, administrated via ingestion of transgenic plants. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：8228 / 8240

页数：13

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