Production of recombinant HIV-1/HBV virus-like particles in Nicotiana tabacum and Arabidopsis thaliana plants for a bivalent plant-based vaccine

被引：46

作者：

Greco, Raffaella ^{[2
]}

Michel, Marie ^{[1
]}

Guetard, Denise ^{[1
]}

Cervantes-Gonzalez, Minerva ^{[1
]}

Pelucchi, Nilla ^{[2
]}

Wain-Hobson, Simon ^{[1
]}

Sala, Francesco ^{[2
]}

Sala, Monica ^{[1
]}

机构：

[1] Inst Pasteur, Dept Virol, F-75015 Paris, France

[2] Univ Milan, Dept Biol, I-20133 Milan, Italy

来源：

VACCINE | 2007年 / 25卷 / 49期

关键词：

HIV-1; HBV; transgenic plants; VLPs; bivalent vaccine;

D O I：

10.1016/j.vaccine.2007.09.061

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Human immunodeficiency virus (HIV-1) and hepatitis B virus (HBV) spread via similar transmission pathways, and infection by HBV occurs in up to 32% of HIV-1 cases. Here, we describe the successful expression of novel recombinant HIV-1/HBV virus-like particles (VLPs) in Nicotiana tabacum and Arabidopsis thaliana. The production levels and quality of the recombinant VLPs were comparable in the two plants, showing that parameters intrinsic to the recombinant proteins determined their assembly into VLPs. These heterologous VLPs can be used in a bivalent anti-HIV-1/-HBV vaccine, administrated via ingestion of transgenic plants. (C) 2007 Elsevier Ltd. All rights reserved.

引用

页码：8228 / 8240

页数：13

共 49 条

[31] In vivo induction of specific cytotoxic T lymphocytes in mice and rhesus macaques immunized with DNA vector encoding an HIV epitope fused with hepatitis B surface antigen
Le Borgne, S
Mancini, M
Le Grand, R
Schleef, M
Dormont, D
Tiollais, P
Rivière, Y
Michel, ML
[J]. VIROLOGY, 1998, 240 (02) : 304 - 315
[32] In vivo induction of cellular and humoral immune responses by hybrid DNA vectors encoding simian/human immunodeficiency virus/hepatitis B surface antigen virus particles in BALB/c and HLA-A2-transgenic mice
Marsac, D
Puaux, AL
Rivière, Y
Michel, ML
[J]. IMMUNOBIOLOGY, 2005, 210 (05) : 305 - 319
[33] Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells
Marusic, Carla
Nuttall, James
Buriani, Giampaolo
Lico, Chiara
Lombardi, Raffaele
Baschieri, Selene
Benvenuto, Eugenio
Frigerio, Lorenzo
[J]. BMC BIOTECHNOLOGY, 2007, 7 (1)
[34] Optimisation of secretion of recombinant HBsAg virus-like particles: Impact on the development of HIV-1/HBV bivalent vaccines
Michel, Marie
Lone, Yu-Chun
Centlivre, Mireille
Roux, Pascal
Wain-Hobson, Simon
Sala, Monica
[J]. VACCINE, 2007, 25 (10) : 1901 - 1911
[35] DNA-MEDIATED IMMUNIZATION TO THE HEPATITIS-B SURFACE-ANTIGEN IN MICE - ASPECTS OF THE HUMORAL RESPONSE MIMIC HEPATITIS-B VIRAL-INFECTION IN HUMANS
MICHEL, ML
DAVIS, HL
SCHLEEF, M
MANCINI, M
TIOLLAIS, P
WHALEN, RG
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (12) : 5307 - 5311
[36] MURASHIGE T, 1962, PHYSIOL PLANTARUM, V15, P493, DOI DOI 10.1111/J.1399-3054.1962.TB08052.X
[37] HIV-1 p24-immunoglobulin fusion molecule: a new strategy for plant-based protein production
Obregon, P
Chargelegue, D
Drake, PMW
Prada, A
Nuttall, J
Frigerio, L
Ma, JKC
[J]. PLANT BIOTECHNOLOGY JOURNAL, 2006, 4 (02) : 195 - 207
[38] A mouse model of human adaptive immune functions:: HLA-A2.1-/HLA-DR1-transgenic H-2 class I-/class II-knockout mice
Pajot, A
Michel, ML
Fazilleau, N
Pancré, V
Auriault, C
Ojcius, DM
Lemonnier, FA
Lone, YC
[J]. EUROPEAN JOURNAL OF IMMUNOLOGY, 2004, 34 (11) : 3060 - 3069
[39] Preserved antigenicity of HIV-1 p24 produced and purified in high yields from plants inoculated with a tobacco mosaic virus (TMV)-derived vector
Pérez-Filgueira, DM
Brayfield, BP
Phiri, S
Borca, MV
Wood, C
Morris, TJ
[J]. JOURNAL OF VIROLOGICAL METHODS, 2004, 121 (02) : 201 - 208
[40] HIV controllers exhibit potent CD8 T cell capacity to suppress HIV infection ex vivo and peculiar cytotoxic T lymphocyte activation phenotype
Saez-Cirion, Asier
Lacabaratz, Christine
Lambotte, Olivier
Versmisse, Pierre
Urrutia, Alejandra
Boufassa, Faroudy
Barre-Sinoussi, Francoise
Delfraissy, Jean-Francois
Sinet, Martine
Pancino, Gianfranco
Venet, Alain
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (16) : 6776 - 6781

← 1 2 3 4 5 →