A combined A431 cell membrane chromatography and online high performance liquid chromatography/mass spectrometry method for screening compounds from total alkaloid of Radix Caulophylli acting on the human EGFR

被引：51

作者：

Sun, Meng ^{[1
,2
]}

Ren, Jing ^{[1
,2
]}

Du, Hui ^{[1
,2
]}

Zhang, Yanmin ^{[1
,2
]}

Zhang, Jie ^{[1
,2
]}

Wang, Sicen ^{[1
,2
]}

He, Langchong ^{[1
,2
]}

机构：

[1] Minist Educ, Key Lab Environm & Genes Related Dis, Beijing, Peoples R China

[2] Xi An Jiao Tong Univ, Sch Med, Xian 710061, Peoples R China

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2010年 / 878卷 / 28期

基金：

中国国家自然科学基金;

关键词：

Cell membrane chromatography (CMC); EGFR; High performance liquid chromatography/mass spectrometry (LC/MS); Radix Caulophylli; RECEPTOR-TYROSINE KINASE; LUNG-CANCER; ANTITUMOR-ACTIVITY; IN-VITRO; INHIBITOR; AFFINITY; MECHANISMS; EXPRESSION; ANTIBODY; TARGETS;

D O I：

10.1016/j.jchromb.2010.08.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We have developed an online analytical method that combines A431 cell membrane chromatography (A431/CMC) with high performance liquid chromatography and mass spectrometry (LC/MS) for identifying active components from Radix Caulophylli acting on human EGFR. Retention fractions on A431/CMC model were captured onto an enrichment column and the components were directly analyzed by combining a 10-port column switcher with an LC/MS system for separation and preliminary identification. Using Sorafenib tosylate as a positive control, taspine and caulophine from Radix Caulophylli were identified as the active molecules which could act on the EGFR. This A431/CMC-online-LC/MS method can be applied for screening active components acting on EGFR from traditional Chinese medicines exemplified by Radix Caulophylli and will be of great utility in drug discovery using natural medicinal herbs as a source of novel compounds. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：2712 / 2718

页数：7

共 35 条

[1] Proteins, drug targets and the mechanisms they control: the simple truth about complex networks [J].

Araujo, Robyn P. ;

Liotta, Lance A. ;

Petricoin, Emanuel F. .

NATURE REVIEWS DRUG DISCOVERY, 2007, 6 (11) :871-880

[2] Mechanisms of angiogenesis and arteriogenesis [J].

Carmeliet, P .

NATURE MEDICINE, 2000, 6 (04) :389-395

[3]

Ciardiello F, 2001, CLIN CANCER RES, V7, P2958

[4] Evidence for the expression of the EGF receptor on human monocytic cells [J].

Eales-Reynolds, LJ ;

Laver, H ;

Mojtahedi, H .

CYTOKINE, 2001, 16 (05) :169-172

[5]

GRANDIS JR, 1993, CANCER RES, V53, P3579

[6] Comprehensive two-dimensional separations of complex mixtures using reversed-phase reversed-phase liquid chromatography [J].

Gray, MJ ;

Dennis, GR ;

Slonecker, PJ ;

Shalliker, RA .

JOURNAL OF CHROMATOGRAPHY A, 2004, 1041 (1-2) :101-110

[7] A brief history of mass spectrometry [J].

Griffiths, Jennifer .

ANALYTICAL CHEMISTRY, 2008, 80 (15) :5678-5683

[8]

He L. C., 1996, NEW PROG BIOMED CHRO, V3, P8

[9] Enzymatic activity and chromatographic characteristics of the cell membrane immobilized on silica surface [J].

He, LC ;

Yang, GD ;

Geng, XD .

CHINESE SCIENCE BULLETIN, 1999, 44 (09) :826-831

[10] Coating and fusing cell membranes onto a silica surface and their chromatographic characteristics [J].

He, LC ;

Wang, SC ;

Geng, XD .

CHROMATOGRAPHIA, 2001, 54 (1-2) :71-76

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