Papa's got a brand new tag: advances in identification of proteases and their substrates

被引：23

作者：

Marnett, AB ^{[1
]}

Craik, CS ^{[1
]}

机构：

[1] Univ Calif San Francisco, Program Chem & Chem Biol, Dept Pharmaceut Chem, San Francisco, CA 94143 USA

来源：

TRENDS IN BIOTECHNOLOGY | 2005年 / 23卷 / 02期

关键词：

D O I：

10.1016/j.tibtech.2004.12.010

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Characterization of proteolytic enzymes and their substrates presents a formidable challenge in the context of biological systems. Despite the fact that an estimated 2% of the human genome codes for proteases, only a small fraction of these enzymes have well-characterized functions. Much of the difficulty in understanding protease biology is a direct result of the complexity of regulation, localization and activation exhibited by this class of enzymes. Here, we focus on several recently developed techniques representing crucial advances toward identification of proteases and their natural substrates.

引用

页码：59 / 64

页数：6

共 19 条

[1] Scanning the prime-site substrate specificity of proteolytic enzymes: A novel assay based on ligand-enhanced lanthanide ion fluorescence [J].

Barrios, AM ;

Craik, CS .

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS, 2002, 12 (24) :3619-3623

[2] A proteomic approach for the discovery of protease substrates [J].

Bredemeyer, AJ ;

Lewis, RM ;

Malone, JP ;

Davis, AE ;

Gross, J ;

Townsend, RR ;

Ley, TJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (32) :11785-11790

[3] Epoxide electrophiles as activity-dependent cysteine protease profiling and discovery tools [J].

Greenbaum, D ;

Medzihradszky, KF ;

Burlingame, A ;

Bogyo, M .

CHEMISTRY & BIOLOGY, 2000, 7 (08) :569-581

[4] Small molecule affinity fingerprinting:: a tool for enzyme family subclassification, target identification, and inhibitor design [J].

Greenbaum, DC ;

Arnold, WD ;

Lu, F ;

Hayrapetian, L ;

Baruch, A ;

Krumrine, J ;

Toba, S ;

Chehade, K ;

Brömme, D ;

Kuntz, ID ;

Bogyo, M .

CHEMISTRY & BIOLOGY, 2002, 9 (10) :1085-1094

[5] Rapid and general profiling of protease specificity by using combinatorial fluorogenic substrate libraries [J].

Harris, JL ;

Backes, BJ ;

Leonetti, F ;

Mahrus, S ;

Ellman, JA ;

Craik, CS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (14) :7754-7759

[6] Taspase1:: A threonine aspartase required for cleavage of MLL and proper HOX gene expression [J].

Hsieh, JJD ;

Cheng, EHY ;

Korsmeyer, SJ .

CELL, 2003, 115 (03) :293-303

[7] Activity-based protein profiling: The serine hydrolases [J].

Liu, YS ;

Patricelli, MP ;

Cravatt, BF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (26) :14694-14699

[8] Protease degradomics:: A new challenge for proteomics [J].

López-Otin, C ;

Overall, CM .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2002, 3 (07) :509-519

[9] Communication between the active sites and dimer interface of a herpesvirus protease revealed by a transition-state inhibitor [J].

Marnett, AB ;

Nomura, AM ;

Shimba, N ;

de Montellano, PRO ;

Craik, CS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (18) :6870-6875

[10] SUBSTRATE PHAGE - SELECTION OF PROTEASE SUBSTRATES BY MONOVALENT PHAGE DISPLAY [J].

MATTHEWS, DJ ;

WELLS, JA .

SCIENCE, 1993, 260 (5111) :1113-1117

← 1 2 →