Enzyme-assisted nanolithography

被引：30

作者：

Riemenschneider, L ^{[1
]}

Blank, S ^{[1
]}

Radmacher, M ^{[1
]}

机构：

[1] Univ Bremen, Inst Biophys, D-28359 Bremen, Germany

来源：

NANO LETTERS | 2005年 / 5卷 / 09期

关键词：

D O I：

10.1021/nl0484550

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

We have chemically immobilized alkaline phosphatase molecules onto the apex of a tip of an atomic force microscope. When the substrate BCIP is dephosphorylated by alkaline phosphatase, it will precipitate in the presence of NBT. By bringing the tip in the vicinity of a suitable sample, we could locally deposit this complex on the sample. Thus we combined the activity of an enzyme with the accuracy in positioning a tip in scanning probe microscopy to demonstrate a novel technique referred to as enzyme-assisted nanolithography. By use of other enzymes, this method will open the possibility to chemically modify surfaces on a nanometer scale.

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页码：1643 / 1646

页数：4

相关论文

共 10 条

[1] Microfabricated devices in biotechnology and biochemical processing [J].

Chován, T ;

Guttman, A .

TRENDS IN BIOTECHNOLOGY, 2002, 20 (03) :116-122

[2] Atomic force microscope imaging of phospholipid bilayer degradation by phospholipase A2 [J].

Grandbois, M ;

Clausen-Schaumann, H ;

Gaub, H .

BIOPHYSICAL JOURNAL, 1998, 74 (05) :2398-2404

[3] Nanolithography using protease etching of protein surfaces [J].

Ionescu, RE ;

Marks, RS ;

Gheber, LA .

NANO LETTERS, 2003, 3 (12) :1639-1642

[4] Functional protein nanoarrays for biomarker profiling [J].

Lynch, M ;

Mosher, C ;

Huff, J ;

Nettikadan, S ;

Johnson, J ;

Henderson, E .

PROTEOMICS, 2004, 4 (06) :1695-1702

[5] Dip-pen nanolithography [J].

Piner, RD ;

Zhu, J ;

Xu, F ;

Hong, SH ;

Mirkin, CA .

SCIENCE, 1999, 283 (5402) :661-663

[6] DIRECT OBSERVATION OF ENZYME-ACTIVITY WITH THE ATOMIC-FORCE MICROSCOPE [J].

RADMACHER, M ;

FRITZ, M ;

HANSMA, HG ;

HANSMA, PK .

SCIENCE, 1994, 265 (5178) :1577-1579

[7] Lithographing of biomolecules on a substrate surface using an enzyme-immobilized AFM tip [J].

Takeda, S ;

Nakamura, C ;

Miyamoto, C ;

Nakamura, N ;

Kageshima, M ;

Tokumoto, H ;

Miyake, J .

NANO LETTERS, 2003, 3 (11) :1471-1474

[8] Protein tracking and detection of protein motion using atomic force microscopy [J].

Thomson, NH ;

Fritz, M ;

Radmacher, M ;

Cleveland, JP ;

Schmidt, CF ;

Hansma, PK .

BIOPHYSICAL JOURNAL, 1996, 70 (05) :2421-2431

[9] Sharpened electron beam deposited tips for high resolution atomic force microscope lithography and imaging [J].

Wendel, M ;

Lorenz, H ;

Kotthaus, JP .

APPLIED PHYSICS LETTERS, 1995, 67 (25) :3732-3734

[10]

WENDEL M, 1996, SUPERLATTICE MICROST, V21, P1